Description

Eukaryotic Elongation Factor 2 Kinase CAS NO 116283-83-1 is a highly specific serine/threonine protein kinase that phosphorylates eukaryotic elongation factor 2 (eEF2), a critical regulator of the peptide chain elongation phase during protein synthesis. This enzyme is a key research target in cellular signaling pathways, particularly those involving nutrient sensing, cell proliferation, and survival. It is essential for biochemical research, drug discovery programs, and academic studies focused on cancer biology, metabolic disorders, and neurological conditions.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study its activity, inhibition, and role in the mTOR and AMPK signaling pathways.
• Drug Discovery & High-Throughput Screening (HTS): Serves as a primary target for screening and validating novel small-molecule inhibitors for potential therapeutic applications in oncology.
• Cell Signaling & Pathway Analysis: Employed to investigate the molecular mechanisms of cellular responses to stress, nutrient availability, and growth factors.
• Academic & Pharmaceutical R&D: Critical for basic research into protein synthesis regulation and for developing targeted therapies against cancers where eEF2K is dysregulated.
• Enzyme Kinetics Studies: Utilized to determine kinetic parameters (Km, Vmax) and elucidate the enzyme's mechanism of action and regulation.
• Production of Phospho-specific Antibodies: Acts as an antigen or tool for generating and validating antibodies that detect phosphorylated eEF2.

Basic Information

Product Name	Eukaryotic Elongation Factor 2 Kinase
CAS No.	116283-83-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	eEF2 Kinase; eEF-2 Kinase; eEF2K; Calcium/calmodulin-dependent protein kinase III; CaM kinase III; CALMK3; Eukaryotic Elongation Factor 2 Kinase; Elongation Factor 2 Kinase; EF-2 Kinase
EINECS	Contact for details

Quality Control

Our Eukaryotic Elongation Factor 2 Kinase is produced under stringent conditions to ensure high activity, purity, and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including activity assays and purity analysis. A detailed Certificate of Analysis (COA) documenting specific activity, purity level, and storage buffer composition is provided with every shipment to guarantee product integrity and support your experimental reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Purity (SDS-PAGE)	> 90%
Specific Activity	> X units/mg protein (Contact for details)
Protein Concentration	X mg/mL (Contact for details)
Buffer Composition	X mM Tris, Y mM NaCl, pH Z, etc.
Endotoxin Level	< 1.0 EU/μg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.