Description

Hydrocortisone-D3 Solution, 10Ppm CAS NO 115699-92-8 is a stable isotope-labeled internal standard solution, specifically formulated for analytical quantification. This product is essential for ensuring the accuracy and reliability of mass spectrometry-based analyses by correcting for instrument variability and sample preparation losses. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and quality control units for precise bioanalytical method development and validation.

Application

• Internal Standard for LC-MS/MS: Critical for the quantitative analysis of hydrocortisone (cortisol) in biological matrices such as plasma, serum, and urine.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate tracking of drug metabolism and concentration-time profiles in clinical and preclinical research.
• Clinical Diagnostics: Used in the development and validation of assays for endocrine disorder diagnosis, including Cushing's syndrome and adrenal insufficiency.
• Doping Control Analysis: Supports the detection and confirmation of exogenous corticosteroid administration in sports anti-doping programs.
• Method Development and Validation: Serves as a key component in establishing robust, reproducible, and GLP-compliant analytical methods.
• Stability-Indicating Assays: Used to monitor the degradation of hydrocortisone in pharmaceutical formulations under various stress conditions.

Basic Information

Product Name	Hydrocortisone-D3 Solution, 10Ppm
CAS No.	115699-92-8
Molecular Formula	C₂₁H₂₇D₃O₅
Molecular Weight	365.48 g/mol
Synonyms	Cortisol-d3; 11β,17α,21-Trihydroxypregn-4-ene-3,20-dione-d3; [2,2,4,6,6,9,12,12,17α-D₉]-11β,17,21-Trihydroxypregn-4-ene-3,20-dione (common labeling pattern); Hydrocortisone (D3); Cortisol Internal Standard (D3); 4-Pregnene-11β,17α,21-triol-3,20-dione-d3; Deuterated Hydrocortisone.
EINECS	Contact for details

Quality Control

Every batch of our Hydrocortisone-D3 Solution is manufactured and tested under strict quality systems. We guarantee high chemical and isotopic purity suitable for sensitive analytical applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as concentration, isotopic enrichment, solvent verification, and absence of interferents. Our quality commitment aligns with the standards expected for GMP/GLP-compliant research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. Due to its highly volatile solvent nature, ensure proper laboratory ventilation during handling and keep the container tightly sealed when not in use to prevent evaporation and concentration change.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	10 µg/mL (10 ppm)
Solvent	Methanol or as specified
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98%
Identification (IR / NMR)	Conforms to structure
Assay (by LC-MS)	95.0 - 105.0% of stated concentration

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.