Description

H-Asn-Amc is a specialized fluorogenic peptide substrate widely used in biochemical and pharmaceutical research. This compound serves as a critical tool for the detection and quantification of protease enzyme activity, offering high sensitivity and specificity. It is essential for researchers and developers in the fields of drug discovery, molecular biology, and diagnostic assay development who require reliable, high-purity biochemical reagents.

Application

• Protease Activity Assays: Primary substrate for fluorometric detection of specific protease enzymes in research settings.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors.
• Enzyme Kinetic Studies: Enables precise measurement of kinetic parameters (Km, Vmax) for enzymatic reactions.
• Diagnostic Development: A key component in developing sensitive diagnostic tests for diseases involving protease dysregulation.
• Biochemical Pathway Analysis: Facilitates the study of proteolytic cascades and signaling pathways in cellular models.
• Quality Control in Biologics: Employed to monitor protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	H-Asn-Amc
CAS No.	115047-89-7
Molecular Formula	C15H18N4O5
Molecular Weight	334.33 g/mol
Synonyms	L-Asparagine 7-amido-4-methylcoumarin; Asparagine-AMC; Asn-AMC; H-Asn-AMC; Asparagine 7-amido-4-methylcoumarin; N-(7-Amino-4-methylcoumarin)asparagine; Fluorogenic substrate Asn-AMC; Protease substrate 115047-89-7
EINECS	Contact for details

Quality Control

Our H-Asn-Amc is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant industry standards for biochemical reagents to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Tested in standard fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.