Description

Clathrin Heavy Chain is a critical structural protein essential for the formation of clathrin-coated vesicles, which are fundamental to intracellular trafficking and receptor-mediated endocytosis. Its high-purity preparation is vital for advancing research in cell biology, neurobiology, and the study of vesicular transport mechanisms. This product is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in proteomics, signaling pathway analysis, and drug discovery.

Application

• Biochemical Research: Used as a key reagent in in vitro studies of clathrin-mediated endocytosis, vesicle formation, and membrane dynamics.
• Proteomics & Structural Biology: Essential for protein-protein interaction studies, complex assembly analysis, and structural determination via techniques like cryo-electron microscopy.
• Drug Discovery Screening: Serves as a target protein in assays designed to identify novel compounds that modulate endocytic pathways, relevant for cancer and neurological disorders.
• Antigen Production: Used to generate specific antibodies for use in immunohistochemistry (IHC), western blotting, and ELISA in diagnostic and research applications.
• Cell Signaling Studies: Employed to investigate the role of clathrin in signal transduction, receptor internalization, and cellular communication.
• Educational & Kit Manufacturing: Supplied to academic institutions for advanced teaching and to manufacturers of research assay kits.

Basic Information

Product Name	Clathrin Heavy Chain
CAS No.	114899-12-6
Molecular Formula	Contact for details
Molecular Weight	~191.5 kDa (monomer)
Synonyms	CLTC; Clathrin Heavy Chain 1; CLH-1; Clathrin Heavy Polypeptide; CHC; Clathrin, Heavy Polypeptide (Hc); Coated Vesicle Membrane Protein; Vesicular Trafficking Protein
EINECS	Contact for details

Quality Control

Our Clathrin Heavy Chain is produced and purified under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot undergoes rigorous quality testing, including purity analysis and functional validation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	≥ 0.5 mg/mL
Identification (Western Blot)	Positive for Clathrin Heavy Chain
Biological Activity (In Vitro Assembly)	Functional
Buffer	Tris-HCl, NaCl, Glycerol, DTT
Endotoxin Level	< 1.0 EU/µg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.