Description

4-Nitrophenyl-N,N',N'',N''',N''''-Pentaacetyl-β-Chitopentaoside is a specialized, chromogenic substrate designed for the sensitive detection and kinetic analysis of chitinase and lysozyme enzymes. This compound matters because it provides a reliable, colorimetric readout for enzymatic activity, enabling precise biochemical research and quality control in industrial enzyme production. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and agricultural biotechnology sectors for studying chitin degradation pathways and developing enzyme-based products. The product is supplied with CAS number 114882-45-0.

Application

• Enzyme Assay Substrate: Primary substrate for the spectrophotometric assay of endo-chitinase and lysozyme activity in research and diagnostic kits.
• Biochemical Research: Used in kinetic studies to determine enzyme inhibition constants (Ki) and catalytic mechanisms of chitinolytic enzymes.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify novel antifungal or antibacterial agents that target chitin synthesis or degradation.
• Agricultural Biotechnology: Applied in the development and quality control of bio-fungicides and transgenic crops engineered for chitinase expression.
• Industrial Enzyme Characterization: Used to standardize and quantify the activity of commercial chitinase preparations used in waste processing or biocatalysis.
• Diagnostic Development: Serves as a key component in developing clinical or environmental diagnostic tests for fungal pathogens.

Basic Information

Product Name	4-Nitrophenyl-N,N',N'',N''',N''''-Pentaacetyl-β-Chitopentaoside
CAS No.	114882-45-0
Molecular Formula	C47H65N5O28
Molecular Weight	1148.04 g/mol
Synonyms	p-Nitrophenyl Pentaacetyl-β-chitopentaoside; PNP-(GlcNAc)5; 4-Nitrophenyl β-D-N,N',N'',N''',N''''-pentaacetylchitopentaoside; Chitinase Substrate PNP-(NAG)5; 4-NP-Pentaacetylchitopentaose; Pentaacetylchitopentaoside 4-nitrophenyl glycoside; 4-Nitrophenyl Penta-N-acetyl-β-chitopentaoside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-N,N',N'',N''',N''''-Pentaacetyl-β-Chitopentaoside is produced under strict quality management protocols to ensure batch-to-batch consistency and high enzymatic reactivity. Each lot undergoes comprehensive analytical testing including HPLC purity analysis, spectrophotometric verification of extinction coefficient, and functional activity testing against standard chitinase. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Specific Optical Rotation	Contact for details
Extinction Coefficient (ε, 305-310 nm)	Contact for details
Functional Activity	≥ 95% of reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.