Description

5'-o-Dimethoxytrityl-O(4)-4-Nitrophenylethyl-2'-o-4-Nitrophenylethylsulfonyluridine is a sophisticated, multi-protected nucleoside derivative designed for advanced oligonucleotide synthesis. This compound is critical for researchers and manufacturers requiring high-precision control over nucleic acid structure and function. It is primarily utilized in the development of therapeutic oligonucleotides, antisense agents, and diagnostic probes, serving the pharmaceutical, biotechnology, and life sciences research sectors.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Acts as a key phosphoramidite precursor for the controlled incorporation of modified uridine residues.
• Therapeutic Oligonucleotide Manufacturing: Essential for producing siRNA, antisense oligonucleotides (ASOs), and aptamers with enhanced stability and specificity.
• Antisense Drug Development: Used in research and GMP-scale production of antisense therapeutics targeting genetic diseases.
• Diagnostic Probe Synthesis: Enables the creation of labeled and modified nucleic acid probes for molecular diagnostics and bioimaging.
• Structural Biology Studies: Facilitates the study of RNA structure, function, and protein-RNA interactions through site-specific modification.
• High-Value Research Chemicals: Supplied to academic and industrial R&D laboratories for exploratory chemistry and novel conjugate development.

Basic Information

Product Name	5'-o-Dimethoxytrityl-O(4)-4-Nitrophenylethyl-2'-o-4-Nitrophenylethylsulfonyluridine
CAS No.	114522-13-3
Molecular Formula	C40H39N3O12S
Molecular Weight	785.82 g/mol
Synonyms	5'-O-DMT-2'-O-(4-Nitrophenylethylsulfonyl)uridine; DMT-2'-O-NPES-Uridine; 2'-O-(2-(4-Nitrophenyl)ethylsulfonyl)-5'-O-(4,4'-dimethoxytrityl)uridine; 5'-O-(4,4'-Dimethoxytrityl)-2'-O-[2-(4-nitrophenyl)ethylsulfonyl]uridine; NPES-protected Uridine phosphoramidite precursor; 114522-13-3; DMT-Uridine(NPES) derivative
EINECS	Contact for details

Quality Control

Our production of this high-purity building block adheres to stringent quality protocols to ensure batch-to-batch consistency for critical research and cGMP applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.