Description

Coelenterazine E is a synthetic, high-purity analog of native coelenterazine, specifically engineered for enhanced stability and performance in bioluminescent applications. This compound serves as a critical substrate for Renilla luciferase (Rluc) and is a cornerstone reagent for advanced cellular and molecular imaging studies. It is essential for researchers and developers in the fields of life sciences, drug discovery, and in vitro diagnostics who require reliable, high-sensitivity luminescent signals for reporter gene assays, high-throughput screening, and live-cell imaging.

Application

• Reporter Gene Assays: Primary substrate for Renilla luciferase (Rluc) in dual-luciferase reporter systems for gene expression analysis.
• Bioluminescence Imaging (BLI): Enables real-time, non-invasive monitoring of biological processes in live cells and small animal models.
• High-Throughput Screening (HTS): Used in cell-based assays for drug discovery to identify modulators of gene expression or signaling pathways.
• ATP Detection & Cell Viability Assays: Employed in kits and assays that utilize luciferase enzymes for quantifying ATP levels.
• Protein-Protein Interaction Studies: Integral to bioluminescence resonance energy transfer (BRET) technologies for studying molecular interactions.
• In Vitro Diagnostic (IVD) Reagent Manufacturing: A key raw material in the production of diagnostic kits and reagents.

Basic Information

Product Name	Coelenterazine E
CAS No.	114496-02-5
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	DeepBlueC; Coelenterazine 400a; 2-Deoxycoelenterazine; 6-(4-Chlorophenyl)-2-[(4-methylphenyl)sulfonyl]-8-phenyl-2H-imidazo[1,2-a]pyrazin-3(7H)-one; Benz[cd]indol-2(1H)-one, 1,1a,6,6a-tetrahydro-6-(4-hydroxyphenyl)-; CTZ-E; Rluc substrate E
EINECS	Contact for details

Quality Control

Our Coelenterazine E is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere. This product is light-sensitive (store away from light) and easily oxidized (store under inert atmosphere). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to pale yellow powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescent Activity	Meets performance criteria in standard Rluc assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.