Description

Fmoc-D-Leucine CAS NO 114360-54-2 is a high-purity, enantiomerically pure amino acid derivative where the α-amino group is protected by a 9-fluorenylmethoxycarbonyl (Fmoc) group. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled, sequential assembly of peptides with defined chirality and sequence. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors who require reliable, high-quality reagents for producing therapeutic peptides, peptide hormones, and complex biomolecules.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a protected D-leucine derivative for the Fmoc/t-Bu strategy, enabling the automated synthesis of custom peptides.
• Pharmaceutical Research & Development: A key intermediate in the synthesis of peptide-based drug candidates, including antimicrobial peptides and metabolic regulators.
• Bioconjugation and Labeling: Used to introduce the D-leucine residue into peptides for creating stable, protease-resistant analogs or for conjugation with other molecules.
• Combinatorial Chemistry Libraries: Serves as a building block for generating diverse peptide libraries for high-throughput screening in drug discovery.
• Academic and Industrial Research: Fundamental reagent for studying protein structure-function relationships, enzyme specificity, and chiral recognition.
• Production of Diagnostic Reagents: Used in the manufacture of peptide antigens and standards for immunoassays and diagnostic kits.

Basic Information

Product Name	Fmoc-D-Leucine
CAS No.	114360-54-2
Molecular Formula	C21H23NO4
Molecular Weight	353.41 g/mol
Synonyms	N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-D-leucine; (R)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-4-methylpentanoic acid; Fmoc-D-Leu-OH; D-Leucine, N-[(9H-fluoren-9-ylmethoxy)carbonyl]-; N-Fmoc-D-leucine; Fmoc-D-leu; (R)-2-(Fmoc-amino)-4-methylpentanoic acid; N-α-Fmoc-D-leucine
EINECS	Contact for details

Quality Control

Our Fmoc-D-Leucine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, meeting the stringent requirements of peptide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and chiral analysis to confirm enantiomeric excess. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key parameters such as assay, optical rotation, and impurity profiles. We support compliance with cGMP and ICH guidelines for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.5%
Optical Rotation [α]20/D	-14.0° to -16.0° (c=1 in DMF)
Chiral Purity (HPLC)	≥99.0% (D-isomer)
Water Content (KF)	≤0.5%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.