Description

6-Chloro-3-Indolyl Acetate is a chromogenic substrate widely used in biochemical and histochemical applications. Its core value lies in its enzymatic cleavage to produce an insoluble, intensely colored indigo derivative, making it an essential tool for detection and localization. This compound is primarily needed by researchers and manufacturers in the life sciences, diagnostic development, and plant biology sectors for applications such as enzyme-linked assays and in-situ staining.

Application

• As a chromogenic substrate for the detection of β-galactosidase and other hydrolytic enzymes in molecular biology (e.g., blue-white screening).
• In histochemistry and cytochemistry for the localization of enzyme activity in tissue sections and cell cultures.
• For the development of diagnostic kits and biosensors requiring a visual or colorimetric readout.
• In plant biology research to study gene expression and promoter activity using GUS (β-glucuronidase) reporter gene systems.
• As a reagent in microbiology for differentiating bacterial colonies based on enzymatic activity.
• In biochemical research for the quantitative assay of esterase and phosphatase enzyme activities.

Basic Information

Product Name	6-Chloro-3-Indolyl Acetate
CAS No.	114305-99-6
Molecular Formula	C10H8ClNO3
Molecular Weight	225.63 g/mol
Synonyms	6-Chloro-3-indolyl acetate; 6-Chloroindol-3-yl acetate; 3-Indolyl 6-chloroacetate; 6-Chloro-1H-indol-3-yl acetate; X-6-Chloroacetate; 6-Cl-3-IAA; Chromogenic substrate for β-galactosidase; 6-Chloro-3-indoxyl acetate
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl Acetate is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, impurities, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.