Description

5-Bromo-4-Chloro-3-Indolyl B-D- is a specialized chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its ability to produce a distinctive, insoluble blue precipitate upon enzymatic cleavage, making it an essential reagent for visualizing and detecting specific enzymatic activities. This compound is critically needed by researchers and manufacturers in the life sciences, particularly for applications in histochemistry, microbiology, and the development of diagnostic kits and assays.

Application

• As a key chromogenic substrate for the detection of β-galactosidase (β-D-galactosidase) activity in molecular biology research, including blue-white screening in cloning.
• In histochemical staining and in situ hybridization techniques to localize gene expression in tissue samples.
• For the development and manufacturing of microbiological culture media and diagnostic plates used in clinical and food safety testing.
• As a critical component in enzyme-linked immunosorbent assays (ELISAs) and other immunoassay formats for signal generation.
• In high-throughput screening (HTS) platforms within pharmaceutical research to identify enzyme inhibitors or activators.
• For quality control and potency testing in the production of recombinant proteins and therapeutic enzymes.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl B-D-
CAS No.	114162-64-0
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; X-Gal; BCIP; 5-Bromo-4-chloro-3-indolyl-β-D-galactoside; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside; Galactoside indoxyl, 5-bromo-4-chloro-; BCIG; Chromogenic β-galactosidase substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl B-D- is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are provided and available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	200 - 205 °C (dec.)
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.