Description

Proto-Oncogene Mbcl-2 Beta Protein CAS NO 114100-40-2 is a high-purity recombinant protein corresponding to a key regulatory domain of the Bcl-2 beta oncoprotein. This protein is essential for fundamental research into the molecular mechanisms of apoptosis (programmed cell death) and cellular survival pathways. It is primarily utilized by researchers and developers in the fields of oncology, molecular biology, and pharmaceutical discovery for applications such as biochemical assays, antibody development, and drug screening.

Application

• Biochemical Research & Assay Development: Used as a standard or target protein in enzyme-linked immunosorbent assays (ELISA), western blotting, and other in vitro studies to investigate protein-protein interactions and signaling pathways.
• Antibody Production & Validation: Serves as a critical immunogen for generating and characterizing monoclonal and polyclonal antibodies specific to the Bcl-2 beta protein domain.
• Drug Discovery & Screening: Acts as a target in high-throughput screening (HTS) platforms to identify and validate novel small-molecule inhibitors or modulators of Bcl-2 family protein interactions for cancer therapy.
• Apoptosis Mechanism Studies: Employed in reconstitution experiments to study the complex regulatory network of pro-apoptotic and anti-apoptotic Bcl-2 family proteins.
• Diagnostic Kit Development: Used as a calibrated reference material in the development of research-use-only (RUO) or diagnostic kits for detecting Bcl-2 expression or related biomarkers.
• Cell Biology & Functional Studies: Utilized in cellular transfection or microinjection experiments to study the functional consequences of Bcl-2 beta protein expression or inhibition.

Basic Information

Item	Detail
Product Name	Proto-Oncogene Mbcl-2 Beta Protein
CAS No.	114100-40-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	B-cell lymphoma 2 beta protein; Bcl-2-beta; BCL2B; BCL2L2; Apoptosis regulator Bcl-2 beta; Recombinant Bcl-2 Beta Protein; Bcl-2β; Bcl-2 beta isoform; Bcl-2 related protein; Anti-apoptotic protein Bcl-2 beta
EINECS	Contact for details

Quality Control

Our Proto-Oncogene Mbcl-2 Beta Protein is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, identity confirmation by mass spectrometry, and functional activity validation where applicable. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure compliance with research-grade standards and support your experimental reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	≥ 90%
Identification (Mass Spec)	Conforms
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Assessed by binding assay (Contact for details)
Protein Concentration	As per COA
Solubility	Soluble in appropriate aqueous buffers
Formulation	Lyophilized from a sterile, preservative-free buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.