Description

Boc-Glu(Obzl)-Ala-Arg-Mca is a specialized, protected tetrapeptide substrate featuring a 7-Methoxycoumarin-4-acetyl (Mca) fluorophore. This compound matters as a critical tool in biochemical research, specifically designed for the sensitive and continuous assay of proteolytic enzyme activity. It is primarily needed by research institutions and pharmaceutical companies engaged in protease inhibitor screening, enzyme kinetics studies, and the development of diagnostic assays.

Application

• Protease Activity Assays: A fluorogenic substrate for the continuous, real-time monitoring of protease activity, particularly for enzymes like trypsin, thrombin, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Enzyme Kinetics & Characterization: Enables the determination of kinetic parameters (Km, Vmax, kcat) for target proteases due to its specific cleavage site.
• Biochemical Research: Serves as a key reagent in fundamental research to study protease function, signaling pathways, and proteolytic processing.
• Diagnostic Development: Potential use in the development of fluorometric diagnostic kits for detecting specific protease levels in clinical samples.

Basic Information

Product Name	Boc-Glu(Obzl)-Ala-Arg-Mca
CAS No.	113866-16-3
Molecular Formula	C₄₀H₅₄N₈O₁₁
Molecular Weight	822.92 g/mol
Synonyms	Boc-Glu(OBzl)-Ala-Arg-MCA; t-Butyloxycarbonyl-γ-benzyl-L-glutamyl-L-alanyl-L-arginyl-4-methylcoumaryl-7-amide; Boc-Glu(OBzl)-Ala-Arg-AMC (common alternate for Mca); Boc-E(OBzl)-A-R-MCA; Fluorogenic Peptide Substrate for Proteases; Protease Substrate V; L-Glutamic acid, N-[(1,1-dimethylethoxy)carbonyl]-5-(phenylmethyl) ester, L-alanyl-N5-(diaminomethylene)-L-ornithyl-7-methoxy-4-methyl-2-oxo-2H-1-benzopyran-3-acetamide
EINECS	Contact for details

Quality Control

Our Boc-Glu(Obzl)-Ala-Arg-Mca is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.