Description

Suc-Ala-Ala-Pro-Val-Amc CAS NO 113277-37-5 is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of elastase and related serine protease activity. This compound is critical for biochemical research and pharmaceutical development, enabling precise enzymatic assays. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical manufacturing sectors.

Application

• Biochemical Research: As a specific fluorogenic substrate for human leukocyte elastase (HLE), porcine pancreatic elastase, and other serine proteases.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential elastase inhibitor compounds for therapeutic development in conditions like chronic obstructive pulmonary disease (COPD) and cystic fibrosis.
• Enzyme Kinetics Studies: Determination of kinetic parameters (Km, Vmax, kcat) for elastase enzymes under various experimental conditions.
• Diagnostic Assay Development: A key component in developing in vitro diagnostic (IVD) kits for measuring elastase activity in clinical or research samples.
• Quality Control in Biologics: Monitoring protease activity and contamination during the production and purification of biopharmaceuticals.
• Inflammation Research: Studying the role of neutrophil elastase in inflammatory pathways and tissue degradation.

Basic Information

Product Name	Suc-Ala-Ala-Pro-Val-Amc
CAS No.	113277-37-5
Molecular Formula	C27H39N5O9
Molecular Weight	577.63 g/mol
Synonyms	N-Succinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin; Suc-AAPV-AMC; Suc-AAPV-AMC Fluorogenic Substrate; Elastase Substrate IV; N-Succinyl-Alanyl-Alanyl-Prolyl-Valyl-7-amido-4-methylcoumarin; Suc-(Ala)3-Pro-Val-AMC; Suc-AAPV-AMC, Fluorogenic; Suc-Ala-Ala-Pro-Val-AMC
EINECS	Contact for details

Quality Control

Our Suc-Ala-Ala-Pro-Val-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay results, and other critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in elastase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.