Description

3,3',5,5'-Tetraethyl-Benzidine Dihydrochloride is a high-purity, substituted benzidine derivative primarily used as a critical chromogenic substrate in enzymatic assays. This compound matters for its role in generating a measurable colorimetric signal, enabling sensitive detection and quantification in diagnostic and research applications. It is essential for manufacturers and R&D laboratories in the in-vitro diagnostic (IVD), clinical chemistry, and life science research sectors who require reliable and consistent performance in their test systems.

Application

• Chromogenic Substrate for Peroxidase (HRP): A key component in ELISA (Enzyme-Linked Immunosorbent Assay) kits and other immunoassays for detecting antigens or antibodies.
• Clinical Diagnostics: Used in automated clinical chemistry analyzers for the detection of various biomarkers, hormones, and infectious agents.
• Research & Development: Employed in biochemical research for studying enzyme kinetics, signal amplification, and developing new diagnostic methodologies.
• Point-of-Care Testing (POCT): Integral to the reagent formulation in rapid test strips and cassettes for on-site diagnostic testing.
• Histology & Cytology: Can be used as a staining reagent in certain immunohistochemical (IHC) and in-situ hybridization techniques.
• Biosensor Development: Serves as a signal-generating molecule in the design of electrochemical and optical biosensors.

Basic Information

Product Name	3,3',5,5'-Tetraethyl-Benzidine Dihydrochloride
CAS No.	112863-92-0
Molecular Formula	C20H30N2·2HCl
Molecular Weight	371.39 g/mol (Free base: 298.47 g/mol)
Synonyms	Tetraethylbenzidine Dihydrochloride; TEB Dihydrochloride; 3,3',5,5'-Tetraethylbenzidine Dihydrochloride; 3,3',5,5'-Tetraethyl-[1,1'-biphenyl]-4,4'-diamine Dihydrochloride; TMB Analog (Tetramethylbenzidine Analog); N,N,N',N'-Tetraethylbenzidine Dihydrochloride; Benzidine, 3,3',5,5'-tetraethyl-, dihydrochloride; Diagnostic Grade TEB
EINECS	Contact for details

Quality Control

Our 3,3',5,5'-Tetraethyl-Benzidine Dihydrochloride is manufactured under strict quality management systems to ensure it meets the exacting standards required for diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, spectrophotometric analysis for performance, and stringent control of moisture content. Certificates of Analysis (COA) with full traceability are provided to guarantee batch-to-batch consistency, high sensitivity, and low background signal in your final assay formulations.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white or light yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Absorbance (Specific Wavelength)	Meets internal criteria for chromogenic performance
Solubility	Soluble in water, methanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.