Description

Substance P, Tyr(0)-(4'-N3)Phe(8)-Nle(11)- CAS NO 112396-65-3 is a chemically modified, photoactivatable analog of the neuropeptide Substance P. This specialized derivative is engineered for advanced research applications, incorporating a photo-leucine (Nle) substitution and a tyrosine-based azide (N3) moiety for crosslinking studies. It is an essential tool for neuroscientists and pharmacologists investigating neurokinin-1 (NK-1) receptor signaling pathways, protein-protein interactions, and receptor localization. The compound's design facilitates covalent binding upon photoactivation, enabling precise, irreversible labeling for proteomic analysis and molecular mapping.

Application

• Neuropharmacology Research: Used as a photoaffinity labeling probe to study the binding site and conformational changes of the Neurokinin-1 (NK-1) receptor.
• Bioconjugation & Proteomics: Serves as a key reagent for "click chemistry" applications, enabling the site-specific attachment of fluorophores, biotin, or other tags to receptor complexes for isolation and analysis.
• Signal Transduction Studies: Facilitates the investigation of Substance P-mediated signaling cascades in pain perception, inflammation, and emotional regulation by allowing irreversible receptor engagement.
• Drug Discovery Platforms: Employed in high-throughput screening assays to identify and characterize novel antagonists or modulators of the tachykinin receptor family.
• Molecular Imaging: Acts as a precursor for developing targeted imaging agents to visualize receptor distribution in cell cultures or tissue sections.
• Peptide Chemistry & Tool Development: Provides a model compound for developing new photoactivatable peptide probes and studying structure-activity relationships (SAR).

Basic Information

Product Name	Substance P, Tyr(0)-(4'-N3)Phe(8)-Nle(11)-
CAS No.	112396-65-3
Molecular Formula	C68H98N20O14S
Molecular Weight	1443.7 g/mol
Synonyms	Substance P (4-azidophenylalanine8, norleucine11); [Tyr0,(4'-N3)Phe8,Nle11]-Substance P; Photoactivatable Substance P analog; Azido-Substance P derivative; Neurokinin-1 receptor photoaffinity ligand; [p-Azido-Phe8,Nle11]-Substance P; SP photo-crosslinking probe
EINECS	Contact for details

Quality Control

Every batch of our photoactivatable peptide is synthesized and purified under cGMP-like conditions to ensure the highest standard of reproducibility and biological activity. Quality is verified through a comprehensive suite of analytical techniques, including HPLC for purity assessment, Mass Spectrometry (MS) for identity confirmation, and Amino Acid Analysis (AAA). A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, peptide content, and performance data relevant to its research application.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. Aliquot into single-use quantities to minimize freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by AAA)
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Acetonitrile/Water mixtures
Biological Activity	Functional activity confirmed in NK-1 receptor binding assay (Data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.