Description

Ethyleneglycol Bis(Succinimidyl Succinate)Hydroxyethylethylenediamine Triacetic Acid is a specialized heterobifunctional crosslinking agent that combines an amine-reactive N-hydroxysuccinimide (NHS) ester with a metal-chelating triacetic acid group. This unique molecular architecture is critical for creating stable, site-specific conjugates between biomolecules and surfaces or other molecules, particularly in applications requiring controlled orientation and minimal activity loss. It is an essential reagent for researchers and manufacturers in the fields of bioconjugation, diagnostics, and advanced material science who require precise and reliable crosslinking chemistry.

Application

• Bioconjugation and Protein Labeling: For covalently attaching proteins, antibodies, or peptides to nanoparticles, surfaces, or other biomolecules via primary amines while offering a chelation site for metals.
• Diagnostic Assay Development: Used in the manufacture of immunoassay components, such as enzyme-antibody conjugates or signal-amplification systems, where stable linkage and specific orientation are paramount.
• Affinity Chromatography Resin Activation: Functionalizing agarose or other matrices for immobilizing metal-binding proteins (e.g., His-tagged proteins) through a stable amine bond.
• Drug Delivery System Fabrication: Enabling the construction of targeted drug carriers by linking therapeutic agents to targeting ligands or carrier polymers.
• Biosensor and Biochip Surface Chemistry: Creating functionalized surfaces for the specific capture of analytes in sensing platforms.
• Materials Science: Modifying polymer surfaces or nanoparticles to introduce metal-chelating properties for catalysis or selective binding applications.

Basic Information

Product Name	Ethyleneglycol Bis(Succinimidyl Succinate)Hydroxyethylethylenediamine Triacetic Acid
CAS No.	112028-42-9
Molecular Formula	C24H32N4O14
Molecular Weight	600.54 g/mol
Synonyms	EGS-HEDTA; Ethylene glycol bis(succinimidylsuccinate)-HEDTA; HEDTA-bis(NHS ester); N1-(2-{[2,5-Dioxo-1-pyrrolidinyl]oxy}-2-oxoethyl)-N1-[2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)ethyl]-N2,N2-bis(2,5-dioxo-1-pyrrolidinyl) succinamide; Heterobifunctional HEDTA-NHS crosslinker; NHS-HEDTA crosslinker; Succinimidyl HEDTA ester
EINECS	Contact for details

Quality Control

Our Ethyleneglycol Bis(Succinimidyl Succinate)Hydroxyethylethylenediamine Triacetic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under desiccant.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (Functional NHS ester)
Solubility	Soluble in DMF, DMSO; partially soluble in water (hydrolyzes)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.