Description

Neurofilament Protein M is a crucial intermediate filament protein that forms the structural scaffold of neuronal axons, essential for maintaining their caliber and mechanical strength. This high-purity protein is vital for advanced research in neurobiology and the development of diagnostic tools for neurological disorders. It serves as a critical reagent for neuroscientists, diagnostic manufacturers, and pharmaceutical researchers focusing on axonal integrity, neurodegeneration, and biomarker discovery.

Application

• Biomarker Research & Assay Development: Used as a standard antigen in immunoassays (ELISA, Western Blot) to detect and quantify neurofilament levels in cerebrospinal fluid (CSF) and blood, a key indicator of neuronal damage.
• Neurodegenerative Disease Studies: Essential for in vitro models researching the pathophysiology of diseases like Amyotrophic Lateral Sclerosis (ALS), Alzheimer's, and Parkinson's disease.
• Antibody Production & Validation: Serves as a highly specific immunogen for generating and characterizing monoclonal and polyclonal antibodies against neurofilament subunits.
• Cell Culture & Neuroscience Models: Utilized in studies of neuronal differentiation, axonal transport, and the cytoskeletal dynamics in primary neuronal cultures or cell lines.
• Diagnostic Kit Manufacturing: A core component in the production of calibrated diagnostic kits for clinical laboratories to assess neuroaxonal injury.
• Toxicology & Neurotoxicity Screening: Applied in assays to evaluate the impact of chemical compounds or pharmaceuticals on neuronal structural proteins.

Basic Information

Product Name	Neurofilament Protein M
CAS No.	111365-29-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Neurofilament Medium Polypeptide; NF-M; 160 kDa Neurofilament Protein; Neurofilament Triplet M Protein; NEFM; NF160; Neurofilament Protein, Medium; Intermediate Filament Protein NEFM
EINECS	Contact for details

Quality Control

Our Neurofilament Protein M is produced and tested under stringent conditions to ensure batch-to-batch consistency and high biological activity for research and diagnostic applications. Each lot is characterized for purity and identity using advanced analytical techniques. A comprehensive Certificate of Analysis (COA) detailing purity, identity (immunoreactivity), and endotoxin levels is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot reconstituted protein to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Immunoblot)	Complies
Purity (SDS-PAGE)	> 95%
Protein Content (BCA)	> 90%
Immunoreactivity (ELISA)	Fully reactive with standard anti-NF-M antibodies
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.