Description

Acetyl Coenzyme A, [Acetyl-3H] is a high-specific-activity, tritium-labeled derivative of the essential metabolic cofactor acetyl-CoA. This radiolabeled compound is critical for tracing and quantifying the flux of acetyl groups in biochemical pathways, enabling precise metabolic research. It is an indispensable tool for researchers in pharmaceutical development, biochemistry, and metabolic disease studies who require accurate, sensitive detection of acetylation reactions and energy metabolism.

Application

• Enzymatic Activity Assays: Used as a labeled substrate for measuring the activity of acetyltransferases (e.g., histone acetyltransferases - HATs) and other acetyl-CoA-utilizing enzymes.
• Metabolic Pathway Tracing: Enables the study of the citric acid cycle (Krebs cycle), fatty acid synthesis, and cholesterol biosynthesis through radiolabel tracking.
• Drug Discovery & Screening: Serves as a key reagent in high-throughput screens for identifying modulators of metabolic enzymes and epigenetic regulators.
• Biochemical Research: Facilitates in vitro studies of protein acetylation, acetylation-dependent signaling pathways, and energy metabolism.
• Radio-HPLC & LC-MS Standards: Acts as a reference standard in chromatographic methods for the identification and quantification of acetylated metabolites.

Basic Information

Product Name	Acetyl Coenzyme A, [Acetyl-3H]
CAS No.	110073-15-9
Molecular Formula	C23H38N7O17P3S (unlabeled parent compound)
Molecular Weight	809.57 g/mol (unlabeled parent compound)
Synonyms	Acetyl-CoA, [acetyl-3H]; [3H]Acetyl-Coenzyme A; Tritiated Acetyl Coenzyme A; Acetyl-CoA (tritiated); Acetyl-S-CoA; Acetyl-Coenzyme A, labeled; Coenzyme A, S-acetate-3H; S-Acetylcoenzyme A-3H
EINECS	Contact for details

Quality Control

Every batch of Acetyl Coenzyme A, [Acetyl-3H] is manufactured and tested under stringent quality protocols to ensure high radiochemical purity and specific activity. Our quality assurance includes rigorous analysis via radio-HPLC and TLC to confirm identity and purity. A comprehensive Certificate of Analysis (COA), detailing specific activity, radiochemical purity, and analytical methods, is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store frozen at or below -20°C in the original solution. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive; protect from all light exposure. For long-term storage, keep at -70°C to -80°C. Aliquot contents to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	Contact for details (Typical range: 1-10 Ci/mmol)
Radiochemical Purity (Radio-HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 90%
Concentration	Contact for details (e.g., 1 mCi/mL, 50 µCi)
Solvent	Aqueous buffer solution (e.g., 50 mM ammonium acetate, pH 4-5)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.