Description

Sulphosuccinimidyl-6-(Biotinamido)Hexanoate is a heterobifunctional crosslinking reagent that combines an amine-reactive N-hydroxysuccinimide (NHS) ester with a biotin moiety. This compound is critically important for the bioconjugation and labeling of proteins, antibodies, and other biomolecules, enabling their detection, purification, and immobilization via the high-affinity biotin-streptavidin interaction. It is an essential tool for researchers and manufacturers in the fields of biotechnology, diagnostics, and pharmaceutical development, facilitating advanced assay development and targeted drug delivery systems.

Application

• Protein and Antibody Biotinylation: Primary application for labeling primary amines (lysine residues) on proteins for use in ELISA, Western blotting, and immunofluorescence.
• Diagnostic Assay Development: Conjugation of detection enzymes (e.g., HRP, alkaline phosphatase) or fluorescent probes to antibodies and antigens for diagnostic kits.
• Affinity Chromatography: Immobilization of biotinylated ligands onto streptavidin/avidin resins for the purification of specific biomolecules.
• Cell Surface Labeling: Tagging of cell surface proteins for flow cytometry (FACS) analysis and cell sorting applications.
• Microarray and Biosensor Fabrication: Site-directed immobilization of capture molecules on sensor chips and microarray slides to ensure proper orientation and activity.
• Drug Conjugate Synthesis: Used in the research and development of antibody-drug conjugates (ADCs) and other targeted therapeutic agents.
• Pull-Down Assays: Identification of protein-protein interactions by biotinylating a bait protein for capture with streptavidin beads.

Basic Information

Item	Details
Product Name	Sulphosuccinimidyl-6-(Biotinamido)Hexanoate
CAS No.	109940-19-4
Molecular Formula	C20H30N4O8S
Molecular Weight	486.54 g/mol
Synonyms	Sulfo-NHS-LC-Biotin; N-Hydroxysulfosuccinimidyl-6-(biotinamido)hexanoate; Biotinamidohexanoic acid sulfo-N-hydroxysuccinimide ester; Biotin-LC-sulfo-NHS; Biotin-XX sulfosuccinimidyl ester; EZ-Link Sulfo-NHS-LC-Biotin; Sulfo-NHS-LC-Biotin, Sodium Salt; 6-((Biotinoyl)amino)hexanoic acid sulfo-N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our Sulphosuccinimidyl-6-(Biotinamido)Hexanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive life science research and in vitro diagnostic (IVD) applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and the active NHS ester is susceptible to hydrolysis. For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (by biotinylation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.