Description

Fmoc-His(Boc)-Opfp CAS NO 109053-20-5 is a high-purity, protected amino acid derivative specifically engineered for solid-phase peptide synthesis (SPPS). This compound is critical for researchers and manufacturers requiring precise and efficient coupling of histidine residues while preventing unwanted side reactions. It is an essential building block for the pharmaceutical, biotechnology, and research sectors involved in developing therapeutic peptides, diagnostic agents, and complex biomolecules.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key Fmoc-protected amino acid active ester (Opfp ester) for the efficient, racemization-free incorporation of Boc-protected histidine into peptide chains.
• Therapeutic Peptide Manufacturing: Used in the research, development, and GMP-scale production of peptide-based pharmaceuticals, including hormones, antibiotics, and targeted therapies.
• Combinatorial Chemistry & Library Synthesis: Serves as a reliable building block for generating diverse peptide libraries for drug discovery and high-throughput screening.
• Bioconjugation & Protein Engineering: Facilitates the site-specific modification of proteins and other biomolecules by introducing histidine residues or tags.
• Academic & Industrial Research: A fundamental reagent in biochemistry and molecular biology labs for studying protein structure-function relationships and designing novel biomaterials.
• Antibody-Drug Conjugate (ADC) Development: Can be utilized in the synthesis of peptide linkers or payloads for targeted cancer therapies.

Basic Information

Product Name	Fmoc-His(Boc)-Opfp
CAS No.	109053-20-5
Molecular Formula	C₃₂H₃₃F₅N₄O₇
Molecular Weight	680.62 g/mol
Synonyms	Fmoc-His(Boc)-OPfp; N-α-Fmoc-N-π-Boc-L-histidine pentafluorophenyl ester; Fmoc-L-histidine(N-π-Boc)-OPfp ester; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonylamino)-3-(1-(tert-butoxycarbonyl)-1H-imidazol-4-yl)propanoic acid 2,3,4,5,6-pentafluorophenyl ester; Fmoc-His(Boc)-O-Pfp; Fmoc-Histidine(Boc)-Opfp; Fmoc-His(Boc)-OPfp Ester; Fmoc-His(Boc)-OPfp Active Ester
EINECS	Contact for details

Quality Control

Our Fmoc-His(Boc)-Opfp is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and cGMP applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This material is hygroscopic (moisture-sensitive) and must be kept under a dry, inert atmosphere. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation [α]²⁰D	Contact for details
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.