Description

Nbd-Demecolcin CAS NO 108964-31-4 is a fluorescently labeled derivative of the microtubule-depolymerizing agent demecolcine, designed for advanced biochemical and cellular research. This compound is a critical tool for visualizing and quantifying microtubule dynamics in live-cell imaging and high-throughput screening applications. It is primarily utilized by researchers in pharmaceutical development, cell biology, and molecular diagnostics who require precise, fluorescent probes for cytoskeletal studies.

Application

• Live-Cell Imaging Probe: Used as a fluorescent marker to track microtubule assembly and disassembly in real-time within living cells.
• High-Throughput Screening (HTS): Serves as a key reagent in assays designed to discover novel anti-mitotic or tubulin-targeting therapeutic agents.
• Biochemical Research: Employed in in vitro tubulin binding studies and fluorescence polarization assays to investigate drug-target interactions.
• Cellular Mechanism Studies: Facilitates the study of cell cycle progression, mitosis, and apoptosis by specifically labeling microtubule networks.
• Diagnostic Development: Potential use in developing diagnostic kits for conditions related to cytoskeletal abnormalities.

Basic Information

Product Name	Nbd-Demecolcin
CAS No.	108964-31-4
Molecular Formula	C24H25N5O5
Molecular Weight	463.49 g/mol
Synonyms	NBD-Demecolcine; 7-Nitrobenz-2-oxa-1,3-diazole Demecolcine; NBD-Colcemid; NBD-Colecmid; Fluorescent Demecolcine; NBD-Labeled Demecolcine; N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-N-desacetyl-N-methylcolchicine
EINECS	Contact for details

Quality Control

Our Nbd-Demecolcin is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and fluorescence properties. We adhere to relevant standards for research-grade biochemicals to support the integrity of your scientific work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or ambient temperatures will lead to degradation. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorescence Properties	Ex/Em ~465/535 nm (in methanol)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.