Description

5-Bromo-4-Chloro-3-Indolyl-A-D-Glucopyranoside is a specialized chromogenic substrate used for the detection of specific enzymatic activity. This compound is critical for research and diagnostic applications where precise and sensitive detection is required. It is primarily utilized by biochemists, molecular biologists, and diagnostic kit manufacturers in life science research and clinical diagnostics.

Application

• As a chromogenic substrate for β-D-glucosidase in enzyme-linked assays and histochemical staining.
• For the detection and quantification of glucosidase activity in microbiological and cell culture studies.
• In the development and manufacturing of diagnostic kits and reagents for clinical and research laboratories.
• As a key component in molecular biology research, particularly in reporter gene assays (e.g., lacZ gene expression).
• For histological and cytological staining to visualize enzyme localization in tissue sections.
• In quality control processes within the biotechnology and pharmaceutical industries to test for specific enzymatic contaminants or activities.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-A-D-Glucopyranoside
CAS No.	108789-36-2
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-α-D-Glucopyranoside; 5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside; BCIP-α-D-Glucoside; X-α-Glc; 5-Bromo-4-chloro-1H-indol-3-yl α-D-glucopyranoside; X-α-D-Glucoside; Chromogenic β-glucosidase substrate; α-D-Glucopyranoside, 5-bromo-4-chloro-3-indolyl
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-A-D-Glucopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.