Description

Neurofilament Protein H is a high-purity, research-grade protein component of the neuronal cytoskeleton, essential for maintaining axonal caliber and structural integrity. Its primary value lies in its critical role as a specific biomarker for neuronal health and damage in neurological research and diagnostic development. This product is indispensable for neuroscience research laboratories, pharmaceutical companies developing neuroprotective therapies, and diagnostic kit manufacturers focusing on conditions like amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and traumatic brain injury.

Application

• Biomarker Research & Assay Development: Used as a standard and critical reagent in the development and validation of immunoassays (ELISA, Western Blot) to detect neurofilament levels in cerebrospinal fluid (CSF) and blood.
• Neurodegenerative Disease Studies: Essential for investigating axonal damage and disease progression in models of ALS, Alzheimer's, Parkinson's, and multiple sclerosis.
• Diagnostic Kit Manufacturing: Serves as a purified antigen for coating plates or generating calibration curves in commercial in-vitro diagnostic (IVD) kits.
• Antibody Production & Validation: Used to immunize animals for generating monoclonal or polyclonal antibodies specific to Neurofilament Heavy Chain.
• Cell Biology & Neuroscience: Employed in studies of neuronal differentiation, axonal transport, and cytoskeletal dynamics in primary cultures or cell lines.
• Toxicology & Drug Safety Screening: Applied in preclinical studies to assess potential neurotoxicity of pharmaceutical compounds or environmental agents.
• Traumatic Brain Injury (TBI) & Stroke Research: Utilized as a key marker to quantify the extent of neuronal injury and monitor recovery in experimental models.

Basic Information

Item	Details
Product Name	Neurofilament Protein H
CAS No.	108688-71-7
Molecular Formula	N/A (Protein)
Molecular Weight	~110-115 kDa (approx., varies by species and post-translational modifications)
Synonyms	Neurofilament Heavy Polypeptide; NF-H; 200 kDa Neurofilament Protein; Neurofilament Triplet H Protein; NEFH; Neurofilament, Heavy Polypeptide; Neurofilament Protein, High Molecular Weight; NF200; Axonal Neurofilament Heavy Chain
EINECS	Contact for details

Quality Control

Our Neurofilament Protein H is produced and purified under stringent conditions to ensure high biological activity and lot-to-lot consistency. Each batch is subjected to rigorous analytical testing, including SDS-PAGE, Western Blot analysis for specificity, and protein concentration determination. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and functional data. Our quality system is designed to support research and diagnostic applications requiring reliable and reproducible protein performance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C and avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Western Blot)	Positive reaction with anti-NF-H antibodies
Purity (SDS-PAGE)	> 90%
Protein Concentration	≥ 1.0 mg/vial (Lot-specific)
Endotoxin Level	< 1.0 EU/μg
Solubility	Soluble in appropriate aqueous buffers
Biological Activity	Confirmed by immunoassay reactivity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.