Description

D-Leu-Ser-Thr-Arg p-Nitroanilide is a synthetic peptide substrate designed for high-precision enzymatic research and diagnostic applications. This compound is critical for studying and quantifying the activity of specific proteases, particularly those involved in blood coagulation and fibrinolysis pathways. It is an essential reagent for pharmaceutical R&D, clinical diagnostics, and biochemical research laboratories requiring reliable and sensitive assay components.

Application

• Protease Activity Assays: Primary substrate for the kinetic study and quantification of thrombin, trypsin, and other serine proteases.
• Diagnostic Reagent Manufacturing: Key component in the production of in-vitro diagnostic (IVD) kits for hemostasis and thrombosis testing.
• Pharmaceutical Research: Used in high-throughput screening (HTS) for the discovery and development of novel anticoagulant and antithrombotic drugs.
• Biochemical Research: Essential tool for investigating enzyme kinetics, inhibition mechanisms, and substrate specificity in academic and industrial settings.
• Quality Control in Biologics: Employed in potency testing and lot-release assays for therapeutic enzymes and related biopharmaceuticals.

Basic Information

Product Name	D-Leu-Ser-Thr-Arg p-Nitroanilide
CAS No.	108321-44-4
Molecular Formula	C24H38N8O9
Molecular Weight	582.61 g/mol
Synonyms	D-Leucine-L-serine-L-threonine-L-arginine 4-nitroanilide; D-Leu-Ser-Thr-Arg-pNA; Thrombin Substrate I (D-Leu); Chromogenic Substrate for Thrombin; Tos-Gly-Pro-Arg-pNA Analog; H-D-Leu-Ser-Thr-Arg-pNA; D-LSTR-pNA; Peptide p-Nitroanilide Substrate 108321-44-4
EINECS	Contact for details

Quality Control

Our D-Leu-Ser-Thr-Arg p-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is subjected to comprehensive analytical testing, including HPLC for purity and spectrophotometric verification of functional activity. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.