Description

Pho2 Regulatory Protein (Sacch Cerev) is a highly purified protein derived from *Saccharomyces cerevisiae* (baker's yeast). This protein plays a critical role in the regulation of phosphate metabolism and gene expression within yeast cells, making it an essential research tool for studying eukaryotic transcriptional control mechanisms. It is primarily utilized by researchers in molecular biology, biochemistry, and biotechnology for applications ranging from fundamental genetic studies to the development of novel bioprocesses.

Application

• Transcriptional Regulation Studies: Used as a key reagent to investigate the mechanisms of phosphate-responsive gene regulation in eukaryotic model systems.
• Protein-Protein Interaction Assays: Employed in pull-down, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens to map interaction networks.
• Biochemical Reagent: Serves as a standard or substrate in enzymatic assays and for studying DNA-binding activities in vitro.
• Educational & Research Kits: Incorporated into laboratory kits for teaching advanced concepts in genetics and cellular signaling pathways.
• Drug Discovery Screening: Potential target for screening compounds that modulate phosphate sensing pathways, relevant to metabolic disorders.
• Bioprocess Development: Study of its regulatory function aids in optimizing yeast-based fermentation and recombinant protein production systems.

Basic Information

Product Name	Pho2 Regulatory Protein (Sacch Cerev)
CAS No.	107853-08-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pho2p; PHO2 Regulatory Protein; Yeast Pho2 Protein; Transcription Factor Pho2; BAS2 Protein; GRF10 Protein; Phosphate Metabolism Regulatory Protein Pho2
EINECS	Contact for details

Quality Control

Our Pho2 Regulatory Protein is produced under stringent conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including SDS-PAGE, HPLC, and functional activity assays. A detailed Certificate of Analysis (COA) documenting purity, identity, and biological activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture absorption. Aliquot reconstituted protein to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single major band
Purity (HPLC)	≥ 90%
Protein Content (Bradford)	≥ 80%
Biological Activity	Confirmed by DNA-binding assay (e.g., EMSA)
Endotoxin Level	< 1.0 EU/μg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.