Description

L-Phenylalaninamide, n-[(2S)-2-(2,5-Dioxo-1-Pyrrolidinyl)-1-Oxopropyl]-L-Alanyl-L-Prolyl-n-(4-Nitrophenyl)- is a specialized synthetic peptide derivative, primarily utilized as a high-value building block and research chemical. Its complex structure, featuring a maleimide-activated N-terminus and a p-nitroanilide (pNA) chromogenic leaving group, makes it a critical reagent for targeted bioconjugation and enzymatic activity studies. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, proteomics, and biochemical assay design, where precise molecular coupling and substrate specificity are paramount.

Application

• Protease Substrate: Acts as a chromogenic substrate for the study and quantification of specific protease enzyme activities, leveraging the p-nitroanilide (pNA) leaving group.
• Bioconjugation Linker: The maleimide functional group enables selective, stable thiol-coupling (Michael addition) for labeling proteins, antibodies, or other biomolecules.
• Pharmaceutical Intermediate: Serves as a key synthetic precursor in the research and development of novel peptide-based therapeutics and diagnostic agents.
• Biochemical Research: Used in academic and industrial R&D for investigating enzyme kinetics, inhibition mechanisms, and signal transduction pathways.
• Assay Development: A critical component in the formulation of high-throughput screening (HTS) assays for drug discovery programs targeting proteases.
• Peptide Synthesis: Employed as a protected or activated fragment in solid-phase peptide synthesis (SPPS) for constructing complex peptide sequences.

Basic Information

Product Name	L-Phenylalaninamide, n-[(2S)-2-(2,5-Dioxo-1-Pyrrolidinyl)-1-Oxopropyl]-L-Alanyl-L-Prolyl-n-(4-Nitrophenyl)-
CAS No.	107409-54-1
Molecular Formula	C30H35N7O8
Molecular Weight	621.65 g/mol
Synonyms	N-[(2S)-2-(2,5-Dioxo-1-pyrrolidinyl)-1-oxopropyl]-L-alanyl-L-prolyl-N-(4-nitrophenyl)-L-phenylalaninamide; Maleimidopropionyl-Ala-Pro-Phe-pNA; Mpr-Ala-Pro-Phe-pNA; Protease Substrate Mpr-Ala-Pro-Phe-pNA; Chromogenic Peptide Substrate; p-Nitroanilide Peptide Derivative; Thiol-Reactive Peptide Substrate; CAS 107409-54-1
EINECS	Contact for details

Quality Control

Our L-Phenylalaninamide derivative is manufactured under strict quality protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research and development applications. All products undergo rigorous analytical testing, including HPLC for purity and identity confirmation. Comprehensive Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with each shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spectrometry)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.