Description

5-Bromo-4-Chloro-3-Indolyl-α-D-Galactopyranoside is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its enzymatic cleavage by α-galactosidase, producing an insoluble, intensely colored indigo derivative ideal for histochemical staining and detection. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and in-vitro diagnostic (IVD) kit production sectors.

Application

• Primary substrate for the detection and assay of α-galactosidase enzyme activity.
• Critical component in microbiological media and chromogenic agars for the specific identification of bacterial colonies (e.g., E. coli).
• Used in histochemistry and cytochemistry for visualizing enzyme localization in tissue sections and cell cultures.
• Key reagent in the development of diagnostic kits and assays for genetic disorders like Fabry disease.
• Employed in molecular biology research for reporter gene assays and gene expression studies.
• Utilized in food safety testing for detecting specific enzyme-producing microorganisms.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-α-D-Galactopyranoside
CAS No.	107021-38-5
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-α-Gal; 5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside; BCIG; α-Galactosidase Substrate; Chromogenic α-Gal Substrate; 5-Bromo-4-chloro-1H-indol-3-yl α-D-galactopyranoside; Indoxyl-α-D-galactoside (bromo-chloro derivative)
EINECS	Contact for details

Quality Control

Every batch of our 5-Bromo-4-Chloro-3-Indolyl-α-D-Galactopyranoside is manufactured and tested under strict quality management systems. We ensure high purity and consistent performance suitable for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, identity, and performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details
Heavy Metals	< 20 ppm
Performance (Enzymatic)	Yields intense blue precipitate with α-galactosidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.