Description

4-Nitrophenyl B-Laminaritrioside is a specialized chromogenic substrate designed for the sensitive detection and quantification of specific enzymatic activity. This compound matters as a critical reagent in biochemical research, enabling precise measurement of enzyme kinetics and inhibition studies. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in carbohydrate enzymology and high-throughput screening.

Application

• Enzymatic Substrate for B-Glucanases: Specifically used as a substrate for endo-1,3(4)-β-glucanases and related enzymes to measure activity via the release of 4-nitrophenol.
• Biochemical Research & Assay Development: A key reagent in developing colorimetric and spectrophotometric assays for studying enzyme mechanisms, kinetics, and inhibitor screening.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for the detection and quantification of specific glucanase enzymes in clinical or environmental samples.
• Plant & Fungal Biology Studies: Applied in research to analyze and characterize β-glucan degrading enzymes in plant pathogens, fungi, and microorganisms.
• Quality Control in Enzyme Production: Employed in industrial settings to standardize and verify the activity of commercial enzyme preparations, such as those used in biofuel or food processing.
• Carbohydrate Chemistry Investigations: Serves as a model compound for studying the hydrolysis of laminarin-type oligosaccharides and related polysaccharides.

Basic Information

Product Name	4-Nitrophenyl B-Laminaritrioside
CAS No.	106678-64-2
Molecular Formula	C30H47NO23
Molecular Weight	765.70 g/mol
Synonyms	p-Nitrophenyl β-laminaritrioside; 4-Nitrophenyl β-D-laminaritrioside; 4-Nitrophenyl β-laminaritrioside; PNPL; pNP-Laminaritrioside; 4-NP-β-laminaritrioside; O-4-Nitrophenyl β-laminaritrioside; 1,3;1,4-β-D-Glucan 4-nitrophenyl trisaccharide derivative
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl B-Laminaritrioside is produced and tested to meet the exacting standards required for research and diagnostic applications. Each batch undergoes rigorous analytical characterization, including HPLC for purity and identity confirmation, to ensure consistent performance and reliability. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.