Description

Immunodominant Protein P35, Vaccinia Virus is a highly purified recombinant protein derived from the Vaccinia virus, a critical tool in virology and immunology research. This protein serves as a key antigen for the study of host immune responses, particularly T-cell recognition, and is essential for vaccine development and immunological assay standardization. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic manufacturers focused on poxvirus research, antiviral drug discovery, and the development of next-generation vaccines.

Application

• Immunological Research: Used as a target antigen in ELISA, Western Blot, and immunoprecipitation assays to study antibody responses.
• Vaccine Development: Serves as a reference standard and immunogen in the research and development of novel Vaccinia and related poxvirus vaccines.
• T-Cell Assays: Critical for in vitro stimulation and detection of virus-specific T-cells in ELISpot and intracellular cytokine staining (ICS) flow cytometry.
• Diagnostic Reagent Production: Employed as a coating antigen or component in the manufacture of diagnostic kits for detecting prior Vaccinia virus exposure or smallpox vaccination.
• Antiviral Screening: Used in high-throughput screening platforms to identify compounds that inhibit viral protein function or host-protein interactions.
• Reference Material: Acts as a purified standard for quality control and assay calibration in biopharmaceutical production and academic laboratories.

Basic Information

Product Name	Immunodominant Protein P35, Vaccinia Virus
CAS No.	106640-44-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Vaccinia Virus P35 Protein; P35 Antigen; VACV P35; Immunodominant Antigen P35; Vaccinia Virus Late Protein P35; 35 kDa Vaccinia Virus Protein; VV P35; Recombinant Vaccinia P35 Protein
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized for identity, purity, and biological activity using advanced analytical techniques. Certificates of Analysis (COA) detailing purity, endotoxin levels, and functional assay data are available upon request. Production follows guidelines suitable for research and in vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~35 kDa
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90% of dry weight
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity (ELISA)	Reactivity confirmed with reference antisera
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.