Description

3,3'-(3,3'-Dimethoxy-4,4'-Diphenylene)Bis(2-Phenyl-5-Veratryltetrazolium Chloride) is a specialized tetrazolium salt derivative, primarily valued as a critical reagent in biochemical and cell biology research. Its core function is to serve as a redox indicator in cell viability and proliferation assays, such as the MTT assay, where it is reduced by mitochondrial enzymes to formazan dyes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors who require precise and reliable tools for metabolic activity measurement.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in colorimetric assays (e.g., MTT, XTT) to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in pharmaceutical R&D and toxicology studies to assess the effects of drug candidates or chemical agents on cell health.
• Microbiological Research: Applied in studies of microbial metabolism and for enumerating viable bacterial or fungal cells.
• Biochemical Research: Serves as an electron acceptor in enzymatic assays to study dehydrogenase and reductase activities.
• Diagnostic Kit Manufacturing: Incorporated as a core component in commercial diagnostic and research kits for standardized testing.
• High-Throughput Screening (HTS): Utilized in automated screening platforms within drug discovery pipelines for rapid assessment of compound libraries.

Basic Information

Product Name	3,3'-(3,3'-Dimethoxy-4,4'-Diphenylene)Bis(2-Phenyl-5-Veratryltetrazolium Chloride)
CAS No.	106629-90-7
Molecular Formula	C46H40Cl2N8O6
Molecular Weight	871.77 g/mol
Synonyms	TV; Tetrazolium Violet; Veratryl Tetrazolium Chloride; 2,5-Diphenyl-3-(α-naphthyl)tetrazolium Chloride (related compound); TV Reagent; Tetrazolium Salt TV; Cell Viability Reagent TV; 3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) dichloride
EINECS	Contact for details

Quality Control

Our 3,3'-(3,3'-Dimethoxy-4,4'-Diphenylene)Bis(2-Phenyl-5-Veratryltetrazolium Chloride) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and reliability for sensitive research applications. Certificates of Analysis (COA) detailing purity, assay, and related substance profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Biological Activity (Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.