Description

γ-Monoglutamyl-Coenzyme F420 is a specialized, naturally occurring coenzyme that plays a critical role in unique redox and electron transfer processes in methanogenic and certain actinobacteria. Its value lies in its function as a low-potential, two-electron transfer agent, making it essential for research and development in novel enzymatic pathways. This compound is primarily needed by researchers in biochemistry, enzymology, and microbiology, as well as by companies developing biocatalysts or exploring methanogen metabolism.

Application

• Biochemical Research: Essential cofactor for studying the enzymatic mechanisms of hydrogenases, formate dehydrogenases, and other F420-dependent enzymes in methanogenic archaea.
• Enzyme Activity Assays: Used as a critical substrate or cofactor in in vitro assays to measure the activity and kinetics of F420-dependent oxidoreductases.
• Metabolic Pathway Studies: Investigation of unique metabolic pathways in microorganisms, including methanogenesis and the biosynthesis of secondary metabolites in Mycobacterium and Streptomyces species.
• Biocatalyst Development: Serves as a key component in the development of novel enzymatic systems for synthetic biology and green chemistry applications.
• Pharmaceutical Research: Potential target or tool in the study of anti-tuberculosis drugs, as F420 is involved in the activation of certain prodrugs within mycobacteria.
• Reference Standard: Used as a high-purity analytical standard for the identification and quantification of F420 coenzymes in complex biological samples via HPLC or LC-MS.

Basic Information

Product Name	γ-Monoglutamyl-Coenzyme F420
CAS No.	106324-58-7
Molecular Formula	C29H35N9O17P2
Molecular Weight	843.60 g/mol
Synonyms	Coenzyme F420-0; 7,8-Didemethyl-8-hydroxy-5-deazariboflavin 5'-phosphate; F420-0; 8-Hydroxy-5-deazaflavin derivative; γ-Glutamyl coenzyme F420; 5-Deazaflavin coenzyme; Electron transfer cofactor F420-0
EINECS	Contact for details

Quality Control

Our γ-Monoglutamyl-Coenzyme F420 is produced and handled under controlled conditions to ensure the highest standards of purity and stability for research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV-Vis)	Spectrum corresponds to reference
Purity (HPLC)	≥ 95% (Area % at 420 nm)
Assay	≥ 90% (by UV absorbance)
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.