Description

Rec β-Ecgf (Human) is a highly purified recombinant human Epidermal Growth Factor (EGF) analog, a critical signaling protein involved in cellular growth, proliferation, and differentiation. This product is essential for advanced life science research and biopharmaceutical development, offering consistent biological activity and high purity. It is primarily utilized by researchers and manufacturers in the fields of cell biology, wound healing studies, and therapeutic protein production.

Application

• Cell Culture & Proliferation Studies: Used as a potent mitogen to stimulate the growth of epithelial cells, fibroblasts, and keratinocytes in vitro.
• Wound Healing Research: Investigated for its role in promoting cell migration, proliferation, and tissue regeneration in preclinical models.
• Signal Transduction Pathways: A key reagent for studying the EGF receptor (EGFR) signaling cascade and related intracellular mechanisms.
• Biopharmaceutical Development: Serves as a reference standard or active ingredient in the development of therapeutic formulations for dermatology and regenerative medicine.
• Diagnostic Assay Development: Employed in the calibration and development of immunoassays (e.g., ELISA) to detect EGF levels in biological samples.
• Tissue Engineering: Incorporated into scaffolds and biomaterials to enhance cell attachment and growth for engineered tissues.

Basic Information

Product Name	Rec β-Ecgf (Human)
CAS No.	105843-51-4
Molecular Formula	C726H1148N204O218S8
Molecular Weight	Approx. 16,300 Da
Synonyms	Recombinant Human Epidermal Growth Factor; rhEGF; β-Urogastrone; Urogastrone-β; hEGF; EGF Human; Epidermal Growth Factor Human Recombinant; CAS 62253-63-8 (for EGF); Hegfl; Receptor Ligand
EINECS	Contact for details

Quality Control

Our Rec β-Ecgf (Human) is manufactured under stringent conditions to ensure the highest standards of purity and biological activity. Each batch is subjected to a comprehensive battery of analytical tests, including identity confirmation, purity assessment, and potency bioassays. A Certificate of Analysis (COA) detailing specifications such as purity (by HPLC), endotoxin levels, and biological activity is provided with every shipment to guarantee traceability and compliance with your research or development requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (HPLC)	≥ 95.0%
Molecular Weight (SDS-PAGE)	Approx. 6.2 kDa (reduced)
Biological Activity (Cell Proliferation)	ED50 ≤ 1.0 ng/ml
Endotoxin (LAL)	< 1.0 EU/μg
Solubility	Soluble in water or PBS (pH 7.2-7.4)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.