Description

25-((7-Chloro-4-Nitrobenz-2-Oxa-1,3-Diazole)Methylamino)-27-Norcholesterol CAS NO 105539-27-3 is a specialized, fluorescently labeled sterol derivative designed for advanced biochemical research and diagnostic applications. This compound is critical for researchers requiring high-precision probes to study cholesterol metabolism, membrane dynamics, and lipid-protein interactions. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic kit manufacturers focused on lipid biology and cellular imaging.

Application

• Fluorescent Labeling Agent: Used as a covalent fluorescent tag for cholesterol and related sterols in complex biological matrices.
• Biochemical Research Probe: Essential tool for studying cholesterol trafficking, localization, and metabolism within live cells and model membranes.
• Diagnostic Assay Development: Serves as a key component in developing fluorescence-based assays for lipid disorders and metabolic studies.
• Membrane Biophysics Studies: Employed to investigate lipid raft formation, membrane fluidity, and sterol-protein interactions using fluorescence microscopy (e.g., FRET, FLIM).
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify compounds that modulate cholesterol biosynthesis or transport pathways.
• Reference Standard: Acts as a certified reference material (CRM) for analytical method development and validation in quality control laboratories.

Basic Information

Product Name	25-((7-Chloro-4-Nitrobenz-2-Oxa-1,3-Diazole)Methylamino)-27-Norcholesterol
CAS No.	105539-27-3
Molecular Formula	C₃₄H₄₅ClN₄O₄
Molecular Weight	621.21 g/mol
Synonyms	NBD-Cholesterol; 25-[N-[(7-Chloro-4-nitro-2,1,3-benzoxadiazol-1-yl)methyl]amino]-27-norcholest-5-en-3β-ol; 27-Nor-25-[N-[(7-chloro-4-nitro-2,1,3-benzoxadiazol-1-yl)methyl]amino]cholest-5-en-3β-ol; NBD Methylamino Cholesterol; 25-NBD Cholesterol; Fluorescent Cholesterol Analog; 7-Chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) labeled cholesterol; NBD-C
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and fluorescence properties. Certificates of Analysis (COA) detailing batch-specific results are available upon request. We adhere to GMP principles for the handling of high-value research chemicals to ensure traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container in a sealed secondary container with an appropriate desiccant. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Ex/Em maxima consistent with literature (e.g., ~465 nm / ~535 nm in lipid membrane)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.