Description

2,3-Bis(4-Methoxyphenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical applications. Its primary value lies in its role as a redox indicator and chromogenic substrate, particularly in cell viability and cytotoxicity assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of metabolic activity is critical.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay Analog): Used as a substrate for mitochondrial dehydrogenases in living cells to produce a measurable formazan dye.
• Cytotoxicity Screening: Employed in high-throughput screening of pharmaceutical compounds and chemicals for toxicological evaluation.
• Microbiological Testing: Applied in assays to determine microbial cell count and metabolic activity.
• Biochemical Research: Serves as an electron acceptor in enzymatic studies and redox reaction monitoring.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research use.
• Antioxidant Activity Assays: Utilized in methods designed to measure the free radical scavenging capacity of samples.

Basic Information

Product Name	2,3-Bis(4-Methoxyphenyl)-5-Phenyltetrazolium Chloride
CAS No.	104497-79-2
Molecular Formula	C27H22ClN4O2
Molecular Weight	469.94 g/mol
Synonyms	2,3-Bis(p-anisyl)-5-phenyltetrazolium Chloride; 2,3-Di(4-methoxyphenyl)-5-phenyl-2H-tetrazolium Chloride; 5-Phenyl-2,3-bis(4-methoxyphenyl)tetrazolium Chloride; Tetrazolium Chloride, 2,3-bis(4-methoxyphenyl)-5-phenyl-; MTT-Tetrazolium Analog; p-Methoxy-substituted Tetrazolium Salt
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Methoxyphenyl)-5-Phenyltetrazolium Chloride is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Solubility	Soluble in DMSO, methanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.