Description

2,3-Bis(4-Ethylphenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical research. Its primary value lies in its role as a redox indicator and a substrate for dehydrogenase enzymes, enabling critical detection and quantification processes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise enzymatic assays and cell viability studies are conducted.

Application

• Cell Viability and Cytotoxicity Assays: Used as a substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Enzymatic Activity Detection: Serves as an electron acceptor for dehydrogenase enzymes, forming a colored formazan product for spectrophotometric analysis.
• Biochemical Research: Employed in studying metabolic pathways, oxidative stress, and antioxidant activity in various biological systems.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to evaluate the efficacy and potential toxicity of drug candidates.
• Microbiology: Applied in assays to assess microbial growth, viability, and metabolic function.
• Diagnostic Kit Manufacturing: Incorporated as a key component in diagnostic kits for clinical and research purposes.

Basic Information

Product Name	2,3-Bis(4-Ethylphenyl)-5-Phenyltetrazolium Chloride
CAS No.	104497-78-1
Molecular Formula	C29H27ClN4
Molecular Weight	467.00 g/mol
Synonyms	2,3-Bis(p-ethylphenyl)-5-phenyltetrazolium chloride; 2,3-Bis(4-ethylphenyl)-5-phenyl-2H-tetrazolium chloride; 2,3-Bis(4-ethylphenyl)-5-phenyl-2H-tetrazol-3-ium chloride; Tetrazolium Chloride, 2,3-Bis(4-ethylphenyl)-5-phenyl-; 2,3-Bis(4-Ethylphenyl)-5-Phenyltetrazolium Chloride; 2,3-Bis(4-ethylphenyl)-5-phenyl-3H-tetrazol-3-ium chloride
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Ethylphenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.