Description

2,3-Di(p-Tolyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical applications. Its primary value lies in its role as a redox indicator and a precursor for formazan dye formation, which is critical for viability and cytotoxicity assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise detection and quantification are paramount.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Microbiological Testing: Employed as an indicator for bacterial growth and metabolic activity in various microbiological media and tests.
• Enzyme Activity Detection: Serves as an electron acceptor in dehydrogenase and reductase enzyme activity assays.
• Cytotoxicity Screening: A critical component in high-throughput screening for drug discovery and toxicology studies.
• Biochemical Research: Utilized in research involving redox reactions, electron transport chains, and oxidative stress.
• Diagnostic Kits: Incorporated into commercial diagnostic kits for clinical and research purposes to detect specific enzymatic activities.

Basic Information

Product Name	2,3-Di(p-Tolyl)-5-Phenyltetrazolium Chloride
CAS No.	104497-77-0
Molecular Formula	C28H23ClN4
Molecular Weight	450.96 g/mol
Synonyms	2,3-Di(4-methylphenyl)-5-phenyl-2H-tetrazolium chloride; DPTT; Tetrazolium Violet; TV; 2,3-Bis(4-methylphenyl)-5-phenyltetrazolium chloride; 5-Phenyl-2,3-di-p-tolyltetrazolium chloride; 2,3-Di-p-tolyl-5-phenyltetrazolium chloride
EINECS	Contact for details

Quality Control

Our 2,3-Di(p-Tolyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive analytical applications. Each lot is tested to confirm identity, purity, and performance in standard assay systems. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Assay (Functional)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.