Description

2,3,5-Tris(p-Tolyl)Tetrazolium Chloride is a specialized tetrazolium salt widely recognized for its role as a vital biochemical reagent. This compound is essential for its ability to serve as a redox indicator, producing a highly colored formazan product upon reduction. It is primarily sought after by research institutions, pharmaceutical developers, and quality control laboratories engaged in cell viability assays, microbiology, and enzymatic studies.

Application

• Cell Viability and Cytotoxicity Assays: A key component in MTT and related assays to measure mitochondrial activity in live cells.
• Microbiological Testing: Used as an indicator for bacterial growth and metabolic activity in various culture media.
• Enzyme Activity Studies: Employed in dehydrogenase and reductase enzyme assays as an electron acceptor.
• Biochemical Research: Serves as a sensitive chromogenic substrate in histochemistry and diagnostic kit development.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms for drug discovery and toxicology.
• Seed Viability Testing: Applied in agricultural sciences to assess seed germination potential and vigor.

Basic Information

Product Name	2,3,5-Tris(p-Tolyl)Tetrazolium Chloride
CAS No.	103538-50-7
Molecular Formula	C28H25ClN4
Molecular Weight	453.0 g/mol
Synonyms	TTTC; TPTZ; 2,3,5-Triphenyl-2H-tetrazolium chloride derivative; Tris(p-methylphenyl)tetrazolium chloride; p-Tolyl tetrazolium chloride; Tetrazolium Red (related); MTT analog; Cell proliferation assay reagent
EINECS	Contact for details

Quality Control

Our 2,3,5-Tris(p-Tolyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, ethanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.