Description

N2-T-Boc-N6-(Biotinamido-6-n-Caproylamido)Lysine n-Hydroxysuccinimide Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and proteomics research. This compound is critical for introducing biotin tags onto proteins, peptides, and other biomolecules, enabling subsequent detection, purification, or immobilization via the strong streptavidin-biotin interaction. It is an essential tool for researchers and manufacturers in pharmaceutical development, diagnostic assay production, and life science research who require reliable, site-specific labeling.

Application

• Proteomics & Biomarker Discovery: Biotinylation of proteins for pull-down assays, mass spectrometry analysis, and protein interaction mapping.
• Diagnostic Kit Development: Conjugation of biotin to antibodies, antigens, or oligonucleotides for use in ELISA, lateral flow assays, and other immunoassays.
• Drug Delivery & Targeting: Functionalization of nanoparticles, liposomes, or therapeutic antibodies with biotin for targeted delivery systems.
• Affinity Purification: Immobilization of biomolecules onto streptavidin-coated beads or columns for high-specificity purification workflows.
• Cell Surface Labeling: Tagging of cell surface receptors and other membrane proteins for flow cytometry or microscopy studies.
• Microarray & Biosensor Fabrication: Creating biotinylated capture probes for immobilization on sensor surfaces or biochips.

Basic Information

Item	Detail
Product Name	N2-T-Boc-N6-(Biotinamido-6-n-Caproylamido)Lysine n-Hydroxysuccinimide Ester
CAS No.	102910-27-0
Molecular Formula	C33H55N5O9S
Molecular Weight	697.88 g/mol
Synonyms	Biotin-LC-LC-NHS Ester; Biotinamidocaproylamido-6-aminocaproyl-N-Boc-Lysine N-Hydroxysuccinimide Ester; Biotin-LC-LC-Boc-Lysine NHS Ester; Nε-(Biotinamido-6-n-Caproylamido)-Nα-Boc-L-Lysine N-Hydroxysuccinimide Ester; Biotin-XX-Boc-Lysine NHS Ester; EZ-Link™ Biotin-XX-Boc-Lysine NHS Ester (analog); Long-chain biotinylation reagent; Boc-Lys(Biotin-XX)-OSu
EINECS	Contact for details

Quality Control

Our N2-T-Boc-N6-(Biotinamido-6-n-Caproylamido)Lysine n-Hydroxysuccinimide Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with detailed specifications to ensure traceability and compliance with research-grade standards. Custom purity grades and bulk specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.