Description

Mouse IL-4 is a high-purity recombinant cytokine essential for immunological research and biopharmaceutical development. This protein plays a critical role in regulating immune responses, particularly in the differentiation of T helper 2 (Th2) cells and B-cell activation. It is a fundamental reagent for researchers and manufacturers in the fields of immunology, cell biology, and therapeutic antibody production, enabling studies on allergic responses, autoimmune diseases, and vaccine development.

Application

• Cell Culture & Differentiation: Used to polarize naive CD4+ T cells into Th2 cells in vitro for immunological studies.
• Bioassay Standard: Serves as a critical standard or stimulant in cell-based bioassays (e.g., CTLL-2, TF-1 proliferation assays) to measure biological activity or potency.
• Antibody Production: Employed as a growth and differentiation factor for B cells in hybridoma technology and monoclonal antibody development.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify novel immunomodulators or anti-inflammatory compounds.
• In Vivo Research: Applied in animal models to study the pathophysiology of asthma, allergies, and other Th2-mediated diseases.
• Diagnostic Development: Utilized as a key component in the development of ELISA kits and other immunoassays for detecting immune markers.
• Stem Cell Research: Supports the differentiation and maintenance of certain hematopoietic cell lineages in culture systems.

Basic Information

Product Name	Mouse IL-4
CAS No.	102619-80-7
Molecular Formula	C600H950N160O190S10 (Approx.)
Molecular Weight	~14-15 kDa (Monomer, glycosylated form)
Synonyms	Interleukin-4 (Mouse); IL-4 (Murine); B-Cell Stimulatory Factor 1 (BSF-1); Lymphocyte Stimulatory Factor 1; T-Cell Growth Factor 2 (TCGF-2); B-Cell Differentiation Factor (BCDF); Mast Cell Growth Factor 2 (MCGF-2); Recombinant Mouse Interleukin-4
EINECS	Contact for details

Quality Control

Our Mouse IL-4 is manufactured under stringent conditions to ensure the highest standards of purity and biological activity. Each batch is subjected to a comprehensive battery of tests, including SDS-PAGE, HPLC, and endotoxin level analysis. A Certificate of Analysis (COA) detailing purity, endotoxin levels, and biological activity (determined by cell proliferation assay) is provided with every shipment to guarantee consistency and reliability for your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, it is recommended to keep the lyophilized product at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 98%
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Determined by cell proliferation assay (e.g., CTLL-2)
Identification (Mass Spec)	Consistent with molecular weight
Solubility	Soluble in water or PBS
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.