Description

n-T-Boc-Val-Leu-Gly-Arg p-Nitroanilide Hydrobromide is a specialized synthetic peptide substrate designed for enzymatic research and biochemical assays. This compound is a critical reagent for studying the activity and kinetics of proteolytic enzymes, particularly serine proteases like thrombin and trypsin. It is primarily utilized by research scientists and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for high-throughput screening and enzyme characterization.

Application

• Enzyme Activity Assays: As a chromogenic substrate for the quantitative determination of serine protease activity, where cleavage releases p-nitroaniline for spectrophotometric detection.
• Drug Discovery & High-Throughput Screening (HTS): Used in pharmaceutical R&D to screen for potential protease inhibitors as therapeutic candidates.
• Biochemical Research: For kinetic studies, enzyme mechanism elucidation, and specificity profiling of various proteases in academic and industrial laboratories.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical analysis of blood coagulation factors and other protease-related pathways.
• Quality Control in Biologics: Employed to monitor and validate the activity and purity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	n-T-Boc-Val-Leu-Gly-Arg p-Nitroanilide Hydrobromide
CAS No.	102601-23-0
Molecular Formula	C30H49BrN10O8
Molecular Weight	733.69 g/mol
Synonyms	Boc-Val-Leu-Gly-Arg-pNA•HBr; t-Boc-Val-Leu-Gly-Arg p-Nitroanilide Hydrobromide; BOC-VLG-R-PNA HBR; Thrombin Substrate I (Chromogenic); Boc-Val-Leu-Gly-Arg-p-Nitroanilide Hydrobromide; N-α-t-Butyloxycarbonyl-L-valyl-L-leucyl-glycyl-L-arginine 4-nitroanilide hydrobromide; Chromogenic Substrate for Thrombin; Protease Substrate 102601-23-0
EINECS	Contact for details

Quality Control

Our n-T-Boc-Val-Leu-Gly-Arg p-Nitroanilide Hydrobromide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to relevant quality guidelines to support our customers' compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.