Description

Fluorescein Mono-β-D-Galactopyranoside is a fluorogenic substrate specifically designed for the detection of β-galactosidase activity. This compound is critical for high-sensitivity enzymatic assays, where its non-fluorescent form is cleaved by the enzyme to release the highly fluorescent fluorescein molecule. It is an essential tool for researchers and quality control professionals in life sciences, pharmaceutical development, and diagnostic manufacturing, enabling precise measurement of enzyme activity in cell-based reporter assays, diagnostic kits, and biochemical research.

Application

• β-Galactosidase Reporter Assays: Widely used as a substrate in molecular and cell biology for monitoring gene expression and promoter activity in transfected cells.
• Diagnostic Kit Manufacturing: Serves as a key component in the production of diagnostic kits for detecting bacterial contamination (e.g., E. coli) via β-galactosidase activity.
• Enzyme Activity & Inhibition Studies: Employed in biochemical research for kinetic studies, high-throughput screening (HTS) of enzyme inhibitors, and characterizing β-galactosidase function.
• Cell Viability & Senescence Assays: Utilized in assays like the senescence-associated β-galactosidase (SA-β-gal) stain to identify senescent cells in aging and cancer research.
• Quality Control in Bioprocessing: Applied in pharmaceutical and biotech manufacturing to monitor and validate the activity of β-galactosidase enzymes used in various processes.
• Microbiology & Food Safety Testing: Acts as a selective substrate in chromogenic/fluorogenic media for the identification and enumeration of specific microorganisms.

Basic Information

Product Name	Fluorescein Mono-β-D-Galactopyranoside
CAS No.	102286-67-9
Molecular Formula	C25H20O12
Molecular Weight	512.42 g/mol
Synonyms	Fluorescein β-D-Galactopyranoside; FMG; 5(6)-Carboxyfluorescein Di-β-D-Galactopyranoside (related); β-Gal-Fluor; Fluorogenic β-Galactosidase Substrate; 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one 6-(β-D-galactopyranoside); FDG (context-specific); β-Galactosidase Substrate, Fluorescein-labeled
EINECS	Contact for details

Quality Control

Our Fluorescein Mono-β-D-Galactopyranoside is manufactured under strict quality control protocols to ensure high purity and consistent performance for sensitive assays. Each batch is tested to confirm identity, purity, and functional activity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as HPLC purity, absorbance/fluorescence profiles, and enzymatic activity. We support compliance with relevant research and diagnostic manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity (Enzymatic Assay)	Conforms to reference
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.