Description

5-Bromo-6-Chloro-3-Indoxyl-3-Acetate is a specialized chromogenic enzyme substrate used in advanced biochemical and diagnostic applications. This compound is critical for the detection and quantification of specific enzymatic activities, particularly β-galactosidase and related hydrolases, due to its ability to produce an insoluble, colored precipitate upon cleavage. It is an essential reagent for researchers and manufacturers in the life sciences, molecular biology, and in-vitro diagnostics (IVD) sectors who require high-purity, reliable substrates for sensitive assays and histochemical staining.

Application

• Histochemical Staining: Used as a substrate for the localization of β-galactosidase activity in tissue sections and cell cultures, producing a distinct colored precipitate.
• Molecular Biology Research: Essential in reporter gene assays (e.g., lacZ gene expression) for monitoring gene expression and promoter activity.
• In-Vitro Diagnostics (IVD): A key component in the formulation of diagnostic kits for enzymatic detection, contributing to high sensitivity and specificity.
• Microbiology: Employed in microbial identification systems to detect specific enzymatic profiles of bacteria.
• Biochemical Assay Development: Serves as a critical reagent in developing and optimizing new enzymatic assay protocols for research and quality control.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify modulators of glycosidase enzyme activity.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl-3-Acetate
CAS No.	102185-48-8
Molecular Formula	C10H7BrClNO3
Molecular Weight	304.52 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl acetate; 5-Bromo-6-chloro-1H-indol-3-yl acetate; BCIA; 5-Br-6-Cl-3-I-Acetate; X-5-Bromo-6-Chloro-3-Indoxyl Acetate; 3-Acetoxy-5-bromo-6-chloroindole; 5-Bromo-6-chloro-3-indoxyl acetate; 102185-48-8
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl-3-Acetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and can provide material tailored to specific research or GMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). For long-term storage, consider storing desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.