Description

Fmoc-Glu(Otbu)-Osu is a high-purity, protected amino acid derivative widely used as a critical building block in solid-phase peptide synthesis (SPPS). Its value lies in providing a reactive N-hydroxysuccinimide (Osu) ester for efficient coupling while offering orthogonal protection for the side chain carboxyl group with a tert-butyl (OtBu) ester and the α-amino group with a fluorenylmethyloxycarbonyl (Fmoc) group. This compound is essential for researchers and manufacturers in pharmaceutical development, biotechnology, and academic research requiring precise and efficient peptide chain assembly.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key reagent for the efficient, stepwise construction of peptide chains using Fmoc-chemistry protocols.
• Pharmaceutical Intermediates: Used in the research and production of therapeutic peptides, including hormone analogs and antimicrobial peptides.
• Bioconjugation: Serves as a linker or spacer molecule for attaching peptides to other biomolecules, surfaces, or carriers.
• Combinatorial Chemistry: Employed in libraries for drug discovery to create diverse arrays of peptide-based compounds.
• Academic & Industrial Research: Fundamental tool for studying protein-protein interactions, enzyme substrates, and structure-activity relationships (SAR).
• Production of Glutamic Acid-Containing Peptides: Specifically facilitates the controlled incorporation of glutamic acid with a protected side chain into peptide sequences.

Basic Information

Product Name	Fmoc-Glu(Otbu)-Osu
CAS No.	101214-22-6
Molecular Formula	C28H31NO8
Molecular Weight	509.55 g/mol
Synonyms	Fmoc-Glu(OtBu)-OSu; Fmoc-Glu(OtBu)-ONSu; N-(9-Fluorenylmethoxycarbonyl)-L-glutamic acid 1-tert-butyl ester N-succinimide ester; N-α-Fmoc-L-glutamic acid γ-tert-butyl ester N-succinimide ester; Fmoc-L-glutamic acid 5-tert-butyl ester 1-(2,5-dioxopyrrolidin-1-yl) ester; (2,5-Dioxopyrrolidin-1-yl) (2S)-5-tert-butoxy-4-{[(9H-fluoren-9-ylmethoxy)carbonyl]amino}-5-oxopentanoate; Fmoc-Glu(OtBu)-OSu ester; Fmoc-Glu(OtBu)-O-NHS
EINECS	Contact for details

Quality Control

Our Fmoc-Glu(Otbu)-Osu is manufactured under strict quality management systems to ensure batch-to-batch consistency and high chemical purity for sensitive synthetic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and chiral integrity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed container to reach room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Optical Rotation [α]D20	-15.0° to -18.0° (c=1 in DMF)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.