Description

n-Glutaryl-Gly-Phe 4-Methoxy-β-Naphthylamide is a specialized synthetic peptide derivative designed as a chromogenic substrate for enzyme activity assays. This compound matters for its high specificity and sensitivity in detecting and quantifying protease activity, particularly from enzymes like cathepsin D. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers for biochemical research, drug discovery, and the development of enzymatic diagnostic tests.

Application

• Biochemical Research: Used as a selective chromogenic substrate for studying the kinetics and inhibition of proteolytic enzymes like cathepsin D and related aspartic proteases.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays to identify and characterize potential protease inhibitor drug candidates.
• Diagnostic Assays: Serves as a key component in the development of in vitro diagnostic (IVD) kits for measuring specific protease activity in biological samples.
• Enzyme Characterization: Facilitates the determination of enzyme specificity, optimal pH, and kinetic parameters (Km, Vmax) in purified enzyme preparations.
• Cell Biology Studies: Applied in assays to monitor protease activity linked to cellular processes such as apoptosis, protein degradation, and lysosomal function.

Basic Information

Product Name	n-Glutaryl-Gly-Phe 4-Methoxy-β-Naphthylamide
CAS No.	100940-58-7
Molecular Formula	C₂₉H₃₄N₄O₇
Molecular Weight	550.61 g/mol
Synonyms	Glutaryl-Glycyl-Phenylalanyl 4-Methoxy-2-naphthylamide; Glutaryl-Gly-Phe-4MβNA; Cathepsin D Substrate; GGP-4MβNA; N-Glutaryl-glycyl-L-phenylalanyl-4-methoxy-2-naphthylamide; L-Phenylalanine, N-[N-[N-(1,5-dioxopentyl)glycyl]-, 4-methoxy-2-naphthalenyl ester; Protease Substrate 100940-58-7
EINECS	Contact for details

Quality Control

Our n-Glutaryl-Gly-Phe 4-Methoxy-β-Naphthylamide is produced under stringent conditions to ensure batch-to-batch consistency and high performance in sensitive assays. Quality is verified through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for research use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.