Description

3-(2-(2-(2-Bromoacetamido)Ethoxy)Ethylcarbamoyl)Proxyl, Free Radical is a stable nitroxide radical compound, specifically a proxyl derivative functionalized with a bromoacetamide group. This molecule is critically important as a versatile spin label and spin trap in electron paramagnetic resonance (EPR) spectroscopy for probing molecular structure, dynamics, and interactions. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and material science sectors for advanced biophysical studies and polymer analysis.

Application

• EPR/Spin Labeling: Covalent attachment to biomolecules (proteins, nucleic acids) via its reactive bromoacetamide group for site-directed spin labeling (SDSL) studies.
• Biophysical Research: Investigation of protein conformation, folding, dynamics, and ligand binding interactions in solution.
• Membrane Studies: Probing lipid bilayer properties, membrane protein topology, and lateral diffusion.
• Polymer Science: Acting as a radical probe for studying polymerization kinetics, polymer chain mobility, and degradation mechanisms.
• Oxidative Stress Analysis: Utilization as a spin trap to detect and quantify short-lived reactive oxygen species (ROS) in biological systems.
• Structural Biology: Providing long-range distance constraints via pulsed EPR techniques (e.g., DEER) for structural modeling.
• Drug Discovery: Screening and characterizing the binding of potential drug candidates to target proteins.
• Material Characterization: Assessing free radical content and distribution in various synthetic materials and composites.

Basic Information

Product Name	3-(2-(2-(2-Bromoacetamido)Ethoxy)Ethylcarbamoyl)Proxyl, Free Radical
CAS No.	100900-39-8
Molecular Formula	C₁₄H₂₄BrN₃O₅
Molecular Weight	394.26 g/mol
Synonyms	3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxy, 3-[[2-[2-[(2-Bromoacetyl)amino]ethoxy]ethyl]carbamoyl]-; 3-(2-(2-(2-Bromoacetamido)ethoxy)ethylcarbamoyl)-PROXYL; 3-(2-(2-(2-Bromoacetamido)Ethoxy)Ethylcarbamoyl)-2,2,5,5-Tetramethylpyrrolidin-1-yloxyl; Bromoacetamido-PROXYL; BrAc-PROXYL; 3-(2-(2-(2-Bromoacetamido)Ethoxy)Ethylcarbamoyl)-PROXYL Nitroxide Radical; 100900-39-8; PROXYL spin label with bromoacetamide linker
EINECS	Contact for details

Quality Control

Our 3-(2-(2-(2-Bromoacetamido)Ethoxy)Ethylcarbamoyl)Proxyl, Free Radical is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and high purity, essential for sensitive spectroscopic applications. Each lot undergoes comprehensive analytical characterization, including EPR spectroscopy to confirm radical integrity and purity. Certificates of Analysis (COA) detailing identity, purity, and specific tests are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (EPR)	Complies
Identification (HPLC)	Complies with reference standard
Purity (HPLC)	≥ 95% (Area %)
Radical Content (EPR)	≥ 90%
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, DMF, ethanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.