Description

Protein Hydrolyzates, Fibronectin is a specialized biochemical preparation derived from hydrolyzed proteins, enriched with the high-molecular-weight glycoprotein fibronectin. This product is essential for its role in promoting cell adhesion, migration, and growth, making it a critical component in advanced cell culture and tissue engineering applications. It is primarily utilized by the biotechnology, pharmaceutical, and advanced biomedical research sectors to support complex in vitro studies and the development of regenerative medicine solutions.

Application

• Cell Culture Substrate Coating: Used to coat cultureware surfaces to enhance the attachment and spreading of anchorage-dependent mammalian cells, including stem cells and primary cell lines.
• Tissue Engineering & Regenerative Medicine: Serves as a bioactive component in scaffolds and hydrogels to mimic the extracellular matrix and support tissue regeneration.
• Wound Healing Research: Employed in studies investigating cell migration and proliferation during the wound repair process due to fibronectin's role in these pathways.
• Diagnostic Assay Development: Utilized as a capture or binding agent in immunoassays and other diagnostic platforms that require specific cell-surface protein interactions.
• Biopharmaceutical Production: Applied in the development and optimization of cell culture processes for the manufacturing of vaccines, monoclonal antibodies, and other therapeutic proteins.
• Neuroscience Research: Facilitates the culture of sensitive neuronal cells by providing a supportive matrix for neurite outgrowth and network formation.

Basic Information

Product Name	Protein Hydrolyzates, Fibronectin
CAS No.	100085-35-6
Molecular Formula	Contact for details
Molecular Weight	High Molecular Weight Glycoprotein (~440 kDa subunit)
Synonyms	Fibronectin; Cold-Insoluble Globulin (CIG); Cell-Surface Fibronectin; Plasma Fibronectin; LETS Protein (Large External Transformation-Sensitive protein); Galactoprotein a; Anti-gelatin Factor; Uronic Acid-containing Protein; FN
EINECS	Contact for details

Quality Control

Our Protein Hydrolyzates, Fibronectin is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and functional activity. Certificates of Analysis (COA) detailing specifications such as protein concentration, biological activity (e.g., cell adhesion assays), and purity (by SDS-PAGE or HPLC) are provided to guarantee consistency and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR/SDS-PAGE)	Conforms to standard
Protein Content (Biuret/BCA)	> 90%
Biological Activity (Cell Adhesion)	Conforms to reference standard
Purity (SDS-PAGE, reduced)	> 95% (Single band ~220-250 kDa under reducing conditions)
Solubility	Soluble in buffered saline or cell culture medium
Endotoxin Level (LAL)	< 1.0 EU/mg
Sterility (for specific grades)	Sterile, 0.2 µm filtered

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.