Description

5-Bromo-4-Chloro-3-Indolylglucuronide is a chromogenic substrate widely used in enzymatic assays for the detection of β-glucuronidase activity. This compound is critical for research and diagnostic applications where precise and sensitive enzyme detection is required. It is primarily utilized by professionals in the life sciences, pharmaceutical research, and clinical diagnostics industries.

Application

• Detection of β-Glucuronidase Activity: A key chromogenic substrate in microbiology and molecular biology for identifying bacterial strains, particularly E. coli, based on GUS gene expression.
• Histochemistry and Cell Biology: Used for in situ localization of β-glucuronidase activity in plant and animal tissue samples.
• Pharmaceutical Research: Employed in high-throughput screening (HTS) assays to identify and characterize inhibitors or activators of β-glucuronidase.
• Diagnostic Kits: An essential component in commercial diagnostic kits for water quality testing and food safety analysis to detect fecal contamination.
• Genetic Engineering: Serves as a reporter substrate in plant molecular biology for monitoring gene expression and transformation efficiency (GUS assay).
• Toxicology Studies: Applied in research to study enzyme activity in various metabolic and detoxification pathways.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolylglucuronide
CAS No.	18656-89-8
Molecular Formula	C14H15BrClNO7
Molecular Weight	424.63 g/mol
Synonyms	X-Glucuronide; 5-Bromo-4-chloro-3-indolyl β-D-glucuronic acid; BCIG; X-GlcA; 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid; 3-Indolyl-β-D-glucuronide, 5-bromo-4-chloro-; Magenta-Gluc (trade name related)
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolylglucuronide is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant quality management standards to support our global clientele in pharmaceutical and life science sectors.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Performance in GUS Assay	Clear blue precipitate formation, low background

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.