Description

Naphthol As-Cl Phosphate CAS NO 18228-16-5 is a specialized biochemical reagent belonging to the class of naphthol AS phosphate derivatives. This compound is a critical chromogenic substrate used in enzymatic detection systems, particularly for alkaline phosphatase (ALP) and other phosphatases. It is essential for researchers and manufacturers in the fields of histochemistry, immunohistochemistry (IHC), and in-situ hybridization, where it enables the precise localization of target antigens or nucleic acids in tissue samples. Its reliable performance makes it a key component for diagnostic assay development and life science research.

Application

• Histochemical Staining: Primary substrate for visualizing alkaline phosphatase activity in tissue sections for research and diagnostic pathology.
• Immunohistochemistry (IHC): Used as a chromogen in enzyme-linked antibody detection systems to produce a visible, localized precipitate.
• In-situ Hybridization: Employed in non-radioactive detection methods to label specific DNA or RNA sequences within cells.
• Diagnostic Kit Manufacturing: A key raw material in the production of clinical diagnostic kits for various diseases and conditions.
• Western Blotting: Can be used as an alternative chromogenic substrate for protein detection on membranes.
• Cell Biology Research: For studying phosphatase activity and localization in cultured cells and experimental models.

Basic Information

Product Name	Naphthol As-Cl Phosphate
CAS No.	18228-16-5
Molecular Formula	C20H15ClNO5P
Molecular Weight	415.76 g/mol
Synonyms	Naphthol AS-CL Phosphoric Acid; 3-Chloro-4-hydroxy-2-naphthanilide phosphate; 3-Chloro-4-hydroxy-2-naphthoic acid anilide phosphate; Naphthol AS-CL phosphate ester; Fast Red TR Salt phosphate; AS-CL phosphate; Phosphoric acid mono-(3-chloro-4-hydroxy-naphthalene-2-carboxylic acid anilide) ester; Naphthol AS-CL phosphoric acid ester.
EINECS	Contact for details

Quality Control

Our Naphthol As-Cl Phosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, which is critical for reproducible experimental and diagnostic results. Each lot is tested to confirm identity, purity, and performance as a chromogenic substrate. A comprehensive Certificate of Analysis (COA) detailing specifications such as assay, impurity profile, and solubility is provided with every shipment. We support compliance with ISO 13485 standards for customers in the in-vitro diagnostic (IVD) manufacturing sector.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in organic solvents (e.g., DMF, DMSO)
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Chromogenic Performance	Passes functional test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.