Description

H-Ala-Onp Hcl CAS NO 17463-53-5 is a protected amino acid derivative, specifically L-alanine p-nitrophenyl ester hydrochloride. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled and efficient formation of peptide bonds. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel peptides, APIs, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key reagent for the coupling of L-alanine residues in automated and manual peptide synthesis protocols.
• Pharmaceutical Intermediates: Serves as a crucial intermediate in the synthesis of peptide-based active pharmaceutical ingredients (APIs) and drug candidates.
• Biochemical Research: Used in academic and industrial laboratories for studying enzyme kinetics, protein-protein interactions, and designing peptide mimetics.
• Protecting Group Chemistry: The p-nitrophenyl (Onp) ester group acts as an activated ester, facilitating amide bond formation under mild conditions.
• Custom Peptide Production: Essential for contract research organizations (CROs) and CDMOs producing custom peptides for therapeutic and diagnostic applications.
• Ligand and Probe Synthesis: Employed in the creation of labeled peptides for imaging, diagnostics, and targeted drug delivery systems.

Basic Information

Item	Details
Product Name	H-Ala-Onp Hcl
CAS No.	17463-53-5
Molecular Formula	C9H11N2O4Cl
Molecular Weight	246.65 g/mol
Synonyms	L-Alanine 4-nitrophenyl ester hydrochloride; H-L-Ala-ONp·HCl; N-(tert-Butoxycarbonyl)-L-alanine 4-nitrophenyl ester; Boc-L-Ala-ONp; Alanine p-nitrophenyl ester hydrochloride; 4-Nitrophenyl L-alaninate hydrochloride; (S)-2-Amino-3-(4-nitrophenoxy)-3-oxopropanoic acid hydrochloride; Peptide coupling reagent for alanine.
EINECS	Contact for details

Quality Control

Our H-Ala-Onp Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm enantiomeric integrity, and identity confirmation via IR and NMR spectroscopy. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Optical Rotation [α]D20	+15.0° to +20.0° (c=1 in DMF)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.