Description

Boc-Ile-Onp CAS NO 16948-38-2 is a protected amino acid derivative, specifically N-(tert-Butoxycarbonyl)-L-isoleucine 4-nitrophenyl ester, widely used as a key building block in peptide synthesis. This compound is essential for the controlled, sequential coupling of amino acids, enabling the production of high-purity peptides for research and pharmaceutical development. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors for the synthesis of therapeutic peptides, peptide hormones, and complex biomolecules.

Application

• Solid-Phase Peptide Synthesis (SPPS): A critical reagent for the efficient coupling of L-isoleucine residues in automated and manual peptide synthesis protocols.
• Solution-Phase Peptide Synthesis: Used in classical solution-phase methods for constructing specific peptide sequences, particularly where sterically hindered amino acids are involved.
• Pharmaceutical Intermediate: Serves as a key intermediate in the research and development of novel peptide-based drugs and active pharmaceutical ingredients (APIs).
• Bioconjugation: Employed in the chemical modification of proteins, antibodies, and other biomolecules to attach functional groups or labels.
• Academic & Industrial Research: A fundamental tool in biochemistry and medicinal chemistry laboratories for studying protein structure-function relationships and designing peptide mimetics.
• Production of Custom Peptides: Used by CROs and CDMOs for the contract manufacturing of bespoke peptides for clients in drug discovery and diagnostics.

Basic Information

Product Name	Boc-Ile-Onp
CAS No.	16948-38-2
Molecular Formula	C₁₇H₂₄N₂O₆
Molecular Weight	352.38 g/mol
Synonyms	N-(tert-Butoxycarbonyl)-L-isoleucine 4-nitrophenyl ester; Boc-L-isoleucine 4-nitrophenyl ester; Boc-Ile-ONp; (S)-2-((tert-Butoxycarbonyl)amino)-3-methylpentanoic acid 4-nitrophenyl ester; N-Boc-L-isoleucine p-nitrophenyl ester; Nα-Boc-L-isoleucine p-nitrophenyl ester; tert-Butyloxycarbonyl-L-isoleucine p-nitrophenyl ester; Boc-Ile-ONp ester
EINECS	Contact for details

Quality Control

Our Boc-Ile-Onp is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive applications like peptide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and chiral analysis to confirm enantiomeric integrity. A detailed Certificate of Analysis (COA) documenting identity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent decomposition. For long-term storage, consider desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Optical Rotation [α]²⁰D	Contact for details
Melting Point	Contact for details
Heavy Metals	<20 ppm
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	<0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.